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Background and purpose: Atrophy of the skeletal system caused by spinal cord injury (SCI) can cause secondary systemic metabolic dysfunction, such as glucose intolerance, type 2 diabetes, and insulin resistance. On the other hand, Sirtun1 (SIRT1) and Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1alpha (PGC-1α) are known factors and related proteins involved in skeletal muscle atrophy. Therefore, the present study aimed to investigate the effect of aerobic training with resveratrol consumption on SIRT1 and PGC-1α levels in the gastrocnemius muscles of rats after spinal cord injury. Materials and methods: 36 male Wistar rats aged eight weeks were randomly placed in 4 groups including control, resveratrol, training, and resveratrol+training (each group n=9). After anesthesia, an incision was made in the midline of the back and over the vertebral ridges. The muscles and lamina of the T9 vertebra were removed without damaging the dura mater, Spinal cord injury was caused by dropping a ten-gram weight from a height of 25 mm on the spinal cord in the T10 segment. After confirmation of SCI, a Resveratrol supplement with a dose of 10 mg/kg was injected intraperitoneally and daily (every morning for 4 weeks), and the rats of other groups were injected with the same amount of saline. The aerobic training was carried out with the help of the weight support system for 4 weeks, 5 sessions per week, each session was 58 minutes and the intensity was 20 m/min. 48 hours after the last training session, the gastrocnemius muscle of right leg of all rats was removed. The SIRT1 and PGC-1α levels in the gastrocnemius muscle were measured by the ELISA method. To analyze the data, one-way analysis of variance and LSD post hoc tests were used at the significance level of P

Background and purpose: Atrophy of the skeletal system caused by spinal cord injury (SCI) can cause secondary systemic metabolic dysfunction, such as glucose intolerance, type 2 diabetes, and insulin resistance. On the other hand, Sirtun1 (SIRT1) and Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1alpha (PGC-1α) are known factors and related proteins involved in skeletal muscle atrophy. Therefore, the present study aimed to investigate the effect of aerobic training with resveratrol consumption on SIRT1 and PGC-1α levels in the gastrocnemius muscles of rats after spinal cord injury. Materials and methods: 36 male Wistar rats aged eight weeks were randomly placed in 4 groups including control, resveratrol, training, and resveratrol+training (each group n=9). After anesthesia, an incision was made in the midline of the back and over the vertebral ridges. The muscles and lamina of the T9 vertebra were removed without damaging the dura mater, Spinal cord injury was caused by dropping a ten-gram weight from a height of 25 mm on the spinal cord in the T10 segment. After confirmation of SCI, a Resveratrol supplement with a dose of 10 mg/kg was injected intraperitoneally and daily (every morning for 4 weeks), and the rats of other groups were injected with the same amount of saline. The aerobic training was carried out with the help of the weight support system for 4 weeks, 5 sessions per week, each session was 58 minutes and the intensity was 20 m/min. 48 hours after the last training session, the gastrocnemius muscle of right leg of all rats was removed. The SIRT1 and PGC-1α levels in the gastrocnemius muscle were measured by the ELISA method. To analyze the data, one-way analysis of variance and LSD post hoc tests were used at the significance level of P<0.05. Results: SIRT1 levels in the gastrocnemius muscle of resveratrol and resveratrol+training groups were significantly higher than the control group (P-value respectively 0.023 and 0.007), but despite the increase in the level of this index in the training group compared to the control group, no significant difference was observed (P=0.399). SIRT1 levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than training group (P=0.038). PGC-1α levels in gastrocnemius muscle of training and resveratrol+ training groups were significantly higher than control group (P-value respectively 0.024 and 0.007), but despite the increase in the level of this index in the resveratrol group compared to the control group, no significant difference was observed (P=0.449). PGC-1α levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than reveratrol group (P=0.023). Conclusion: These results show the positive effect of resveratrol consumption, on SIRT1 level, as well as aerobic exercise on PGC-1α level of the biceps muscle in rats with SCI. It has been pointed out that the increase in SIRT1 expression and the subsequent increase in PGC-1α leads to an increase in the number of myonuclei, which improves the recovery process after injury and plays an active role in muscle hypertrophy by upregulating and reducing catabolic processes. herefore, it appears that a combination of aerobic training and resveratrol supplementation can mitigate muscle atrophy caused by spinal cord injury in muscles below the level of injury by influencing the levels of muscle SIRT1 and PGC-1α. [ABSTRACT FROM AUTHOR]

BACKGROUND AND OBJECTIVE: SIRT1 and PGC-1α are two factors that increase the body's antioxidant capacity, which can improve inflammation and free radicals in obesity. The present study was conducted to determine the effect of eight weeks of water training on SIRT1, PGC-1α and body fat percentage in obese men. METHODS: In this quasi-experimental study, obese men (BMI = 30, waist-to-height ratio (WHtR) > 0.5) were selected voluntarily and were randomly divided into two groups of water training (n = 11) and control (n = 11). The training program included three sessions per week for eight weeks with training intensity of 60 to 80% of maximal heart rate. BMI, body fat percentage and weight of subjects were examined before and after the intervention. Arterial blood samples were used to measure SIRT1 and PGC-1α during the pre-test and post-test stages. FINDINGS: The assessments showed that PGC-1α levels increased from 7.7 to 8.7 and SIRT1 levels increased from 11.3 to 12.6, and this increase was significant in the training group compared to the control group (p < 0.001). The results showed that body fat percentage decreased from 30.2 to 27.83 and weight decreased from 94.7 to 92.2, which was significant compared to the control group (p

Background: SIRT1 and FGF21 are known to regulate glucose metabolism and moderate diabetes complications. Sargassum oligocystum extract has therapeutic characteristics. This study aimed to evaluate the effects of hydroalcoholic extract of sargassum oligocystum on serum levels of SIRT1 and FGF21in diabetic rats. Materials and methods: In this experimental study, 48 male Wistar rats were randomly assigned into six groups: the non-diabetic control, the diabetic control, the diabetic treated with 150mg/kg of the extract, the diabetic treated with 300mg/kg of extract, the diabetic treated with 450mg/kg of the extract and the diabetic treated with 100mg/kg of Metformin. After 30 days of treatment, serum levels of SIRT1 and FGF21 of rats were measured. The data was analyzed in SPSS software version 22. Results: The extract of sargassum at the dose of 450mg/kg significantly reduced the SIRT1 serum level, but no changes were observed in the serum level of FGF21 and insulin at any of the doses. Moreover, serum glucose and insulin resistance were decreased at the doses of 300mg/kg of the extract. Conclusion: The results of this study suggest that the algae extract did not significantly change SIRT1 and FGF21 levels in order to regulate the glucose metabolism.

هدف پژوهش حاضر تعیین تأثیر هم‌زمان و مکمل‌دهی رزوراترول و فعالیت هوازی بر میزان پروتئین SIRT-1 ، PGC-1α عضلة نعلی و بافت چربی شکمی‌-کشاله‌ای و UCP-1 بافت چربی شکمی‌-کشاله‌ای در موش‌های صحرایی نر بود. در این پژوهش، 32 سر موش صحرایی(میانگین وزن10 ± 260 گرم، سن هشت هفته)، به‌صورت تصادفی به چهار گروه شاهد، تمرین، مکمل-تمرین و مکمل تقسیم شدند. گروه‌های تمرینی به‌مدت 12 هفته (‌هفته‌ای پنج جلسه، هر جلسه به‌مدت 45 دقیقه) روی نوارگردان فعالیت کردند. گروه مکمل-تمرین روزانه 10 میلی‌گرم به‌ازای هر کیلوگرم از وزن بدنشان مکمل رزوراترول دریافت کردند. برای اندازه‌گیری پروتئین بافتی UCP-1، SIRT1 و PGC-1α از روش الایزا (ساندویچ دوتایی) استفاده‌ شد. آزمون تحلیل واریانس یک‌طرفهبرای تحلیل داده‌ها استفاده شد و سطح معنا‌داری (P≤0.05) درنظر گرفته ‌شد. نتایج نشان داد که میزان پروتئین‌های SIRT1 و PGC-1α در بافت عضله و چربی سفید زیرپوستی در گروه تمرین- مکمل و گروه مکمل، به‌ترتیب با P≤0.001 و P≤0.05 افزایش معناداری داشته است. پروتئین UCP-1 نیز در بافت چربی سفید، پس از تمرینات افزایش معناداری را نشان داد(P≤0.001). با توجه به نتایج به‌دست‌آمده، احتمالاً مکمل‌دهی رزوراترول به‌همراه فعالیت‌بدنی هوازی تأثیر قوی‌تری بر افزایش میزان SIRT-1 و PGC-1α عضلة نعلی و بافت چربی شکمی‌-کشاله‌ای و UCP-1 بافت چربی شکمی‌-کشاله‌ای نسبت به استفادة جداگانه از فعالیت‌بدنی یا مکمل رزوراترول دارد و احتمالاً موجب تغییر فنوتیپ چربی سفید زیرپوستی به چربی بژ (بینابینی) می‌شود.

Abstract Background: Oxidative Stress and mitochondrial dysfunction leading to apoptotic death of neurons play key role in the pathogenesis of the Parkinson's disease. On the other hand، misregulation of microRNAs can cause several neurodegenerative diseases. Sirt1 and Bcl2 as two key genes, regulate pathogenic processes in neurodegenerative diseases such as mitochondrial dyfunction, oxidative stress and apoptosis. Materials and Methods: To investigate the role of microRNAs in model of Parkinson's disease, miRWalk 2.0 and TargetScan (v7) databases were served to predict microRNA-target interactions. Results: Possible targeting effects of different microRNAs on Bcl2 and Sirt1 genes in Rat organism were analyzed. Merging data from databases has shown that rno-miR-449a, rno-miR-182, rno-miR-211, rno-miR-34b, rno-miR-34c, rno-miR-448, rno-miR-466b and rno-miR-96 with strong possibility can inhibit expression of Bcl2 gene. Also, rno-miR-181, rno-miR-211, rno-miR-27a, rno-miR-449a, rno-miR-34c, rno-miR-30, rno-miR-200a and rno-miR-448can inhibit Sirt1 gene with high possibility. Conclusion: According to the findings, it can be predicted that regarding to high interaction scores of rno-miR-211, rno-miR-34c and rno-miR-448 and 449awith Bcl2 and Sirt1 genes in above-mentioned databases, these microRNAs probably can have critical role in disease process. Thus, these microRNAs can be introduced as appropriate candidates for investigations in in vitro model of Parkinson's disease.

Introduction: Sirtuin 1 is a protein deacetylase that plays an important role in many cellular processes. The serum sirtuin 1 levels are higher in the polycystic ovary syndrome (PCOS) patients than the healthy subjects. The aim of this study was to assess the role of SIRT-1 rs7895833 polymorphism in the susceptibility of PCOS. Methods: This case-control study was done on 456 Iranian women from March to February 2015. Blood samples were collected from 274 women with PCOS and 182 age matched (±5) healthy women at Shiraz Ghadir Mother and Child Hospital. After DNA extraction, SIRT1 rs7895833 genotype determination was done by Tetra-ARMS PCR. Data analysis was performed by SPSS version 18. Results: In the dominant model for G allele (AG+GG vs. AA), AG+GG genotypes in SIRT1 rs7895833 gene polymorphism was associated with the increased risk of PCOS (OR: 2.01, %95CI:1.32-3.24, P=0.002). Moreover, there was a significant association between the G allele and PCOS (OR: 1.45, %95CI:1.10-1.91, P=0.008). Conclusion: The results showed a possible association between SIRT1 rs7895833 gene polymorphism and susceptibility to PCOS.

Background & Objectives: Breast cancer is the most common cancer and the second leading cause of cancer deaths among women. Obesity, hormones, and growth factors are the risk factors for this kind of cancer. One of the changes observed in patients suffering from breast cancer is the elevated Visfatin or nicotinamide phosphoribosyl transferase (NAMPT) in their tumor tissues and blood. The increased activity of Visfatin and SIRT1 (Sirtuin 1) in breast cancer and many other cancers has been determined, and its value is correlated with cancer prognosis. The aim of the present study is to investigate the effects of Visfatin on SIRT1 activity in MCF-7 breast cancer cell line. Materials & Methods: In this study, in order to investigate the effects of Visfatin on SIRT1 activity in MCF-7 cells, cells were treated after cell culture by Visfatin for 12, 24, and 48 hours. Subsequently, the cells were lysed by nuclear extraction kit, and their total protein concentrations were measured by Bradford assay. Finally, we estimated the general activity of SIRT1 by measuring the SIRT1 activity with the assay kit via spectrofluorometric device. Results: The findings of this research show that SIRT1 activity is not significantly changed following Visfatin treatments for 12 and 24 hours. However, after 48 hour, Visfatin increases SIRT1 activity about 2 times more than control group. Conclusion: The antiapoptotic effects of Visfatin are exerted by increasing SIRT1 activity in MCF-7 cells, and these effects happen after 24 hours.

Background & Objectives: Breast cancer is the most common cancer and the second leading cause of cancer deaths among women. Obesity, hormones, and growth factors are the risk factors for this kind of cancer. One of the changes observed in patients suffering from breast cancer is the elevated Visfatin or nicotinamide phosphoribosyl transferase (NAMPT) in their tumor tissues and blood. The increased activity of Visfatin and SIRT1 (Sirtuin 1) in breast cancer and many other cancers has been determined, and its value is correlated with cancer prognosis. The aim of the present study is to investigate the effects of Visfatin on SIRT1 activity in MCF-7 breast cancer cell line. Materials & Methods: In this study, in order to investigate the effects of Visfatin on SIRT1 activity in MCF-7 cells, cells were treated after cell culture by Visfatin for 12, 24, and 48 hours. Subsequently, the cells were lysed by nuclear extraction kit, and their total protein concentrations were measured by Bradford assay. Finally, we estimated the general activity of SIRT1 by measuring the SIRT1 activity with the assay kit via spectrofluorometric device. Results: The findings of this research show that SIRT1 activity is not significantly changed following Visfatin treatments for 12 and 24 hours. However, after 48 hour, Visfatin increases SIRT1 activity about 2 times more than control group. [ABSTRACT FROM AUTHOR]