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Soil and plants in natural ecosystems interact with each other. The different structures and characteristics of plant species in an area in the long term affect environmental variables, especially on the soil. This study aimed to investigate the effect of two plant species, Astragalus myriacanthus, and Acantholimon spinosum, on soil chemical properties in different spatial locations. Soil sampling was done in early fall 2018 by randomly selecting 20 replicates (10 individuals of each species) from four locations (upward slope edge, downward slope edge, center, and outside (as control)) in sycamore rangelands Naz, Yazd province. A total of 80 soil samples were collected. Then, soil chemical properties, including pH, EC, total organic matter (TOM), particulate organic matter (POM), and total nitrogen (TN) were measured in the laboratory. The effect of plant presence on soil properties showed that the parameters of organic matter (mean 1.66%), total nitrogen (mean 0.22%), electrical conductivity (mean 368.97 Ms/cm), and POM (with an average of 1.28%) in beneath A. myriacanthus were significantly higher than the soil outside the floor (with averages of 0.92%, 0.13%, Ms/cm 139.5, 58, respectively (P <0.05). TN and POM in the upslope edge were significantly higher than other edges. hr A. spinosum, there were no significant differences of TOM (1.77%), TN (0.23%), EC (215.46 Ms/cm), and pH (7.97) in beneath with outside the canopy. However, the POM with an average of 1.49% had the highest amount under the canopy relative to the outside soil and other sampling sides. In general, this study expresses the different roles of different cushion plants on the chemical and qualitative characteristics of the soil, which by recognizing this role can help improve, develop and maintain their habitats. [ABSTRACT FROM AUTHOR]