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At present, replant disorder and peach (Prunus persica (L.) Batsch) short life has become an important problem in peach production, and it has seriously restricted the development of the peach industry in Chahar Mahal va Bakhtiary province. During 2014-2017, 108 samples of plant tissues and rhizosphere were collected from the peach orchards of this province. Samples were investigated to isolation and identification microorganisms and pathogens associated with symptoms of peach replant problem. Symptoms including: yellowing, stunting, abscission of leaf, leaf discoloration and chlorosis, crown and root rot, canker and death of young trees. From plant and soil samples, the frequency respectively, isolates of Bacillus sp, Fusarium sp, Pseudomonas flourescens, Mesocriconema xenoplax, Aspergillus sp, Alternaria sp, Pantoea agglomerans, Arthrobacter sp, Phytophthora sp, Pseudomonas syringae, Pseudomonas putidae, Verticillium dahliae, Dematophora necatrix, Trichoderma sp, Streptomyces sp, Meloidogyne sp, Pratylenchus sp, Paratylenchus sp, Stenotrophomonas maltophilia, Pythium sp and Rhizoctonia sp were isolated and identified. About 11% of isolates were unknown. The amount of replant disorder and isolation of these microorganisms in orchards that were planted immediately after the destruction of previous trees or planted the same previous species was about 30% more than the orchards that had not been cultivated (fallow) for at least one year. On this basis canbe say these microorganisms are the most important agents associated with replant problem and life short in peach orchards in Chahar Mahal va Bakhtiary province. Koch's postulates investigations are necessary to determine the role of these microorganisms in this replant disease. [ABSTRACT FROM AUTHOR]