Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Introduction: Working in different and night shifts directly affects the health of workers. This situation can be experienced by pilots in civil aviation due to its highly complex and exhausting activity. Purpose: This study aims to investigate the work and sleep factors associated with musculoskeletal symptoms in Brazilian pilots of civil aviation, as well as identify the main body parts affected. Methods: A descriptive epidemiological study was held with a large sample of 1234 pilots from ABRAPAC, who voluntarily replied to a survey from November 2013 to March 2014. The survey had information about age, gender, marital status, work, health and sleep. The dependent variables were the musculoskeletal symptoms and for this, a modified Brazilian version of the Standardized Nordic Questionnaire was used. Also in the Robust Poisson regression, the variables for chronic (in the last 12 months), acute (in the last 7 days) and work-related musculoskeletal symptoms were dichotomized into ¿without symptom¿ and ¿with symptom¿. It was considered ¿with symptom¿ the pilot who reported pain in at least one of the four body parts with the highest prevalence in the study: upper back, lower back, neck and shoulder. The data were analyzed by Stata 12.0 program. Results: The prevalence of chronic musculoskeletal symptoms was 65,9% and work-related 46,9% to the last 12 months. The prevalence of acute musculoskeletal symptoms was 28,0% and work-related 23,3% to the last 7 days. It was verified that pilots who worked 11-21 years , with 66-hour flight journey or more per month , with irregular-work hours involving the night-shifts , with low sleep quality and insomnia were risk factors for chronic musculoskeletal symptoms. These variables were also considered risk factors for work-related symptoms . To acute musculoskeletal symptoms, it was observed that working seven consecutive days or more per month, with low sleep quality and insomnia were risk factors. These variables were also considered risk factors for work-related symptoms . Conclusion: It was concluded that work organizational structure associated with a low sleep quality and insomnia were predisponent factors to musculoskeletal symptoms among Brazilian pilots of civil aviation. The upper and lower back, neck and shoulder were considered the body parts with the highest prevalence. Introdução: O trabalho em turnos e noturno afeta diretamente a saúde do trabalhador, situação essa vivenciada pelos pilotos da aviação civil. Além disso, é uma atividade desgastante, dada a sua alta complexidade, podendo trazer prejuízos à saúde. Objetivo: Analisar a prevalência e os fatores organizacionais e de sono associados aos sintomas musculoesqueléticos entre pilotos da aviação comercial, bem como as regiões corporais de maior prevalência dos sintomas. Métodos: Foi realizado um estudo transversal com os pilotos associados da Associação Brasileira de Pilotos da Aviação Civil - ABRAPAC. Dos 2530 pilotos associados, um total de 1234 pilotos participou voluntariamente da pesquisa respondendo o questionário online, no período de novembro de 2013 a março de 2014. O questionário continha perguntas sobre condições sociodemográficas, trabalho, saúde, estilo de vida e sono. Os desfechos do estudo foram os sintomas musculoesqueléticos avaliados por meio do Questionário Nórdico de Kuorinka, adaptado e validado para a cultura brasileira. Para a análise de regressão de Poisson, com variância robusta, dos sintomas musculoesqueléticos crônicos (nos últimos 12 meses e nos últimos 12 meses relacionados ao trabalho) e agudos (nos últimos sete dias e nos últimos sete dias relacionados ao trabalho) foram dicotomizados em ¿sem sintoma¿ e ¿com sintoma¿. Foi classificado com sintoma o piloto que relatou dor em pelo menos uma das quatro regiões corporais de maior prevalência na amostra estudada, sendo essas, superior das costas, inferior das costas, pescoço e ombros. Em todos os testes foi considerado significante o valor de p