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ABSTRACT: Lamb diarrhea seriously restricts the development of the sheep industry. Infectious pathogens often cause diarrhea, and E. coli isolates are highly distributed in infectious diarrhea. The study aimed to investigate the prevalence of pathogenic E. coli in diarrhea lambs and determine the distribution of virulence genes, antibiotic resistance genes, and antibiotic resistance. One hundred seventy-eight E. coli isolates were isolated from the feces of 204 diarrhea lambs. The virulence genes mdh, hlyF, iss, ompA, fimC, iucD, st, lt, stx1, stx2, and antibiotic resistance genes including tetA, tetB, aac (6′)-II, blaCMY-2, qnr, aadA1, sul1, blaTEM, blaCTX-M were detected by polymerase chain reaction (PCR). Antibiotic resistance was determined by Kirby-Bauer Disc Diffusion method. There were 109 (61.24%, 109/178) enterotoxigenic E. coli (ETEC), 119 (66.85%, 119/178) Shiga toxin-producing E. coli (STEC), and 95 (53.37%, 95/178) hybrid STEC/ETEC isolates. The highest prevalent virulence genes were ompA (80.90%, 144/178) and fimC (67.98%, 121/178), and the lowest was iucD (8.99%, 16/178). The most commonly detected antibiotic resistance genes were tetA/tetB (92.70%, 165/178), qnr (75.84%, 135/178), and sul1 (62.92%, 112/178), no blaTEM or blaCTX-M genes were detected. All isolates had high antibiotic resistance to lincomycin (96.63%, 172/178), tetracycline (88.76%, 158/178), and co-trimoxazole (80.34%, 143/178), and the multidrug resistant (MDR) rate reached 93.82% (167/178). The high prevalence of ETEC and STEC indicates that E. coli is one of the critical pathogenic agents leading to diarrhea in lambs in the region, and its high antibiotic resistance, especially MDR, should be brought to our attention.

This article draws on Bayat’s discussions of the “quiet encroachment” and “social nonmovement” to explore the intertwined relationships between mundane lives, public culture, and spatial orders. It takes the collective singing practices in public parks in the city of Guangzhou in South China as a case study, and at the center of analysis are senior urban residents who have experienced double marginalization under the new political economy and the new spatial order in public spaces. I show that collective singing in parks, which is a cultivated practice acting upon a body’s habitus shaped by the political culture, has contributed to rebuild the social life of the senior citizens after layoffs. While such an embodied practice has been re-appropriated by the government and officials to capitalize on the authority of political culture to further their ends, such re-appropriation also empowers the choral participants to pursue happiness in their familiar ways and legitimize their claims on public spaces that are increasingly coded with a middle-class civility. I argue that the analytical lens offered by “social nonmovement” helps shift attention from agency to action. By focusing on how effects of state-orchestrated governing strategies have been made complicated by ordinary people’s “quiet encroachment”, this article shows that the exercise and effects of power shall be analyzed as a dynamic process with a spatial and temporal dimension. The exploration of the layers of social, political and visceral experiences between individual citizens and the social-political space can effectively help us diagnose how power works and morphs.