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Fundamentos: O nível de metilação global do ADN de leucócitos no sangue tem sido associado ao risco de doença arterial coronariana (DAC), com resultados inconsistentes em diferentes populações. Faltam dados semelhantes da população chinesa, onde diferentes fatores genéticos, de estilo de vida e ambientais podem afetar a metilação do ADN e sua relação com o risco de DCC. Objetivos: Analisar se a metilação global está associada ao risco de doença coronariana na população chinesa. Métodos: Foram incluídos um total de 334 casos de DCC e 788 controles saudáveis. A metilação global do ADN de leucócitos de sangue foi estimada por meio da análise das repetições do LINE-1 usando pirosequenciamento de bissulfito. Resultados: Em uma análise inicial restrita aos controles o nível do LINE-1 diminui significativamente com a idade avançada, colesterol total elevado, e diagnóstico de diabetes. Na análise de caso-controle, a redução da metilação do LINE-1 foi associada ao aumento do risco de DCC, tendo a análise por quartil revelado uma odds ratio (IC 95%) de 0,9 (0,6-1,4), 1,9 (1,3-2,9) e 2,3 (1,6 3.5) para o terceiro, segundo e primeiro (o mais baixo) quartil (P da tendência < 0,001), respectivamente, em comparação com o quarto (o mais alto) quartil. A metilação inferior (< mediana) do LINE-1 esteve associada a 2,2 vezes (IC 95% = 1,7-3,0) o aumento de risco de doença coronariana. As estimativas de risco de DCC menores relacionadas com o LINE-1 tenderam a ser mais fortes entre os indivíduos com maior tercil de homocisteína (P interação = 0,042) e naqueles com diagnóstico de hipertensão arterial (P interação = 0,012). Conclusão: A hipometilação do LINE-1 está associada ao risco de doença coronariana na população chinesa. Fatores de risco de DCC potenciais tais como a idade avançada, colesterol total elevado, e diagnóstico de diabetes podem ter impacto na metilação global do ADN, exercendo, portanto, o seu efeito sobre o risco de doença coronariana.

Atualmente no Brasil, as decisões no gerenciamento das decolagens de aeronaves dependem exclusivamente da experiência do controlador, que, empiricamente, programa um sequenciamento de partidas. Propõe-se então, através do conceito de Tomada Colaborativa de Decisão (CDM), a elaboração de um sistema computacional para o Gerenciamento Colaborativo de Partidas em aeroportos (CoDMAN) com a utilização da Teoria dos Jogos. Neste modelo, cada agente representa uma aeronave a impactar o cenário aeroportuário e suas decisões são fundamentadas na negociação em cenários dinâmicos multiagentes para a consecução de sequências eficientes de decolagens. Um protótipo foi desenvolvido e foram elaboradas simulações com base em planos de voo válidos para o Aeroporto Internacional de Brasília. A interface com os sistemas radar foi simulada com o tratamento dos voos que impactaram a TMA-Brasília em fevereiro de 2013. Os resultados obtidos foram satisfatórios. As otimizações no sequenciamento das decolagens reduziram o custo associado aos atrasos em até 28%, comprovando a eficácia da modelagem proposta.

Nesta pesquisa propõe-se um sistema para sincronização e gerenciamento de fluxo de tráfego aéreo que combina tecnologias de Inteligência Artificial – coordenação de Multi-Agentes, e computação distribuída sobre a Internet – Grid Computacional – cuja finalidade é otimizar o fluxo de tráfego aéreo, prevendo congestionamentos e racionalizando a utilização dos recursos dos aeroportos, sem contudo, ferir os princípios de segurança estabelecidos para a área de transportes aéreos. Para demonstrar o sistema desenvolvido, são descritos sua arquitetura, seus componentes e os relacionamentos entre eles, bem como os mecanismos de negociação necessários às tomadas de decisão. A título ilustrativo, é relatado um estudo de caso de planejamento tático, simulado a partir de informações do Aeroporto Internacional de São Paulo. A combinação das duas tecnologias, coordenação de Multi-Agentes e Grid Computacional, aplicada ao problema de gerenciamento do fluxo de tráfego aéreo apresenta perspectivas de tornar-se um tópico promissor para pesquisas futuras.

Este trabalho descreve um sistema de auxílio à decisão com metodologias de Teoria dos Grafos e Inteligência Artificial para dar suporte ao Gerenciamento de Fluxo de Tráfego Aéreo Brasileiro. Trata-se de um modelo de gerenciamento de fluxo baseado em grafos com adaptações heurísticas para a regulação dinâmica do fluxo. O modelo fundamenta a arquitetura do Módulo de Balanceamento de Fluxo (MBF) integrante do Sistema Distribuído de Apoio a Decisão aplicado ao Gerenciamento Tático do Fluxo de Tráfego(SISCONFLUX), em desenvolvimento, e tem o objetivo de melhorar o gerenciamento do espaço aéreo nacional. O MBF foi proposto para dar suporte ao sistema em operação no Primeiro Centro Integrado de Defesa Aérea e Controle de Tráfego Aéreo (CINDACTA I), fornecendo informações adicionais para o processo aplicado pelos controladores a fim de amenizar a carga de trabalho e melhorar os resultados de suas ações. Usando técnicas de maximização de fluxo adaptados da Teoria dos Grafos, o MBF foi desenvolvido como um modelo de análise que determina o tempo de separação entre decolagens a partir das terminais integrantes da Região de Informação de Vôo de Brasília (FIR-BS) e distribui a folga do fluxo ao longo do espaço aéreo controlado, objetivando prevenir ou reduzir o congestionamento nos diversos setores da FIR-BS. O MBF dá suporte à regulação do fluxo de tráfego auxiliando os controladores e as outras unidades dentro do SISCONFLUX.

Este trabalho descreve um sistema de auxílio à decisão com metodologias de Teoria dos Grafos e Inteligência Artificial para dar suporte ao Gerenciamento de Fluxo de Tráfego Aéreo Brasileiro. Trata-se de um modelo de gerenciamento de fluxo baseado em grafos com adaptações heurísticas para a regulação dinâmica do fluxo. O modelo fundamenta a arquitetura do Módulo de Balanceamento de Fluxo (MBF) integrante do Sistema Distribuído de Apoio a Decisão aplicado ao Gerenciamento Tático do Fluxo de Tráfego(SISCONFLUX), em desenvolvimento, e tem o objetivo de melhorar o gerenciamento do espaço aéreo nacional. O MBF foi proposto para dar suporte ao sistema em operação no Primeiro Centro Integrado de Defesa Aérea e Controle de Tráfego Aéreo (CINDACTA I), fornecendo informações adicionais para o processo aplicado pelos controladores a fim de amenizar a carga de trabalho e melhorar os resultados de suas ações. Usando técnicas de maximização de fluxo adaptados da Teoria dos Grafos, o MBF foi desenvolvido como um modelo de análise que determina o tempo de separação entre decolagens a partir das terminais integrantes da Região de Informação de Vôo de Brasília (FIR-BS) e distribui a folga do fluxo ao longo do espaço aéreo controlado, objetivando prevenir ou reduzir o congestionamento nos diversos setores da FIR-BS. O MBF dá suporte à regulação do fluxo de tráfego auxiliando os controladores e as outras unidades dentro do SISCONFLUX.

Based on the Swedish Standard Industrial Classification and individual longitudinal data, by using the shift-share analysis method, Swedish regional employment growth during the 1990s has been decomposed into two components—growth due to an endogenous regional factor and growth due to an exogenous structural factor. The regional factor is more important in shaping regional growth than the structural factor as indicated by employment, although the two factors both have a positive relation to regional growth. The structural factor creates a macro milieu for a region and the regional factor generates a micro milieu for industries inside the region. The response of core regions to the economic downturn and recovery in Sweden in the period studied is stronger than that of periphery regions. The findings from this study support endogenous growth theories and core-periphery theory.

Nesta pesquisa propõe-se um sistema para sincronização e gerenciamento de fluxo de tráfego aéreo que combina tecnologias de Inteligência Artificial – coordenação de Multi-Agentes, e computação distribuída sobre a Internet – Grid Computacional – cuja finalidade é otimizar o fluxo de tráfego aéreo, prevendo congestionamentos e racionalizando a utilização dos recursos dos aeroportos, sem contudo, ferir os princípios de segurança estabelecidos para a área de transportes aéreos. Para demonstrar o sistema desenvolvido, são descritos sua arquitetura, seus componentes e os relacionamentos entre eles, bem como os mecanismos de negociação necessários às tomadas de decisão. A título ilustrativo, é relatado um estudo de caso de planejamento tático, simulado a partir de informações do Aeroporto Internacional de São Paulo. A combinação das duas tecnologias, coordenação de Multi-Agentes e Grid Computacional, aplicada ao problema de gerenciamento do fluxo de tráfego aéreo apresenta perspectivas de tornar-se um tópico promissor para pesquisas futuras.

The composite electrode material of manganese oxide supported on poly glucose (PGS) was prepared by using KMnO4 as manganese source. In order to study the influence of the support on the structure and properties of the composite electrode materials, the changes of the composition of the composite electrode materials were investigated in different weight ratio of KMnO4/PGS (3:1, 3:3, 3:5, 3:7, 3:9). The characterization results of XRD, Raman special and XPS indicated the composition of manganese oxides was greatly influenced by the content of PGS. When the PGS content is lower, the composition of manganese oxide in hybrid material is single-component oxide (MnOOH or Mn3O4). With the increasing PGS content, a mixture of Mn3O4 and MnCO3 was observed, furthermore proportion of MnCO3 in mixture also increased, and up to higher PGS content (KMnO4/PGS=3:9), single-component MnCO3 was founded on hybrid material. In addition, the change of composition of hybrid materials also led to an obvious difference in electrochemical performance. With the increase of PGS content, the specific capacitance of hybrid materials increases firstly, and reaches the maximum with the weight ratio of KMnO4/PGS equal to 3:3, and then gradually decreases. This was attributed to the higher electrochemical performance of Mn3O4 than that of MnOOH and MnCO3.

Background: Sustained pathological cardiac hypertrophy (CH) is an independent risk factor for increased incidence and mortality of cardiovascular events., Objectives: This research was designed to unravel the role of long non-coding RNA (LncRNA) CCAT2 in CH progression., Methods: Transverse aortic constriction (TAC) procedures were conducted to construct a pressure overload-induced in vivo CH model. Angiotensin II (Ang II) treatment was utilized to induce hypertrophic rat cardiomyocyte H9c2 cells., Results: In vivo results showed that silencing of CCAT2 reduced cardiomyocyte surface area, alleviated cardiac fibrosis, and decreased β-MHC, ANP, and BNP levels in CH mouse models. In vitro results revealed that CCAT2 knockdown reduced cell surface area and attenuated β-MHC, ANP, and BNP levels in hypertrophic H9c2 cells. Besides, CCAT2 silencing decreased the levels of active β-catenin, phosphorylated-GSK-3β, and Wnt target genes (c-Myc, cyclinD1, and c-Jun) in CH mice and hypertrophic H9c2 cells. Importantly, treatment with the Wnt/β-catenin pathway activator LiCl reversed the suppression of CCAT2 knockdown on H9c2 cell surface area and MHC, ANP, and BNP levels., Conclusions: Collectively, CCAT2 silencing plays a protective role against CH through inactivating the Wnt/β-catenin signaling, which suggests that CCAT2 might become a promising therapeutic target for CH.

Background: The anthracycline chemotherapeutic antibiotic doxorubicin (DOX) can induce cumulative cardiotoxicity and lead to cardiac dysfunction. Long non-coding RNAs (lncRNAs) can function as important regulators in DOX-induced myocardial injury., Objective: This study aims to investigate the functional role and molecular mechanism of lncRNA OXCT1 antisense RNA 1 (OXCT1-AS1) in DOX-induced myocardial cell injury in vitro., Methods: Human cardiomyocytes (AC16) were stimulated with DOX to induce a myocardial cell injury model. OXCT1-AS1, miR-874-3p, and BDH1 expression in AC16 cells were determined by RT-qPCR. AC16 cell viability was measured by XTT assay. Flow cytometry was employed to assess the apoptosis of AC16 cells. Western blotting was used to evaluate protein levels of apoptosis-related markers. Dual-luciferase reporter assay was conducted to verify the binding ability between miR-874-3p and OXCT1-AS1 and between miR-874-3p and BDH1. The value of p<0.05 indicated statistical significance., Results: OXCT1-AS1 expression was decreased in DOX-treated AC16 cells. Overexpression of OXCT1-AS1 reversed the reduction of cell viability and promotion of cell apoptosis caused by DOX. OXCT1-AS1 is competitively bound to miR-874-3p to upregulate BDH1. BDH1 overexpression restored AC16 cell viability and suppressed cell apoptosis under DOX stimulation. Knocking down BDH1 reversed OXCT1-AS1-mediated attenuation of AC16 cell apoptosis under DOX treatment., Conclusion: LncRNA OXCT1-AS1 protects human myocardial cells AC16 from DOX-induced apoptosis via the miR-874-3p/BDH1 axis.

to examine the relationship between career success and work environment of nurses who practice in assisted reproductive technology and to identify factors that affect career success. a cross-sectional study conducted in 53 fertility centres in 26 provinces in mainland China. Data were collected using a demographic data questionnaire, a specialised nursing competence questionnaire, the Career-Success Scale, and the Nursing Work Environment Scale. Descriptive and inferential statistics were applied. 597 assisted reproductive technology nurses participated in our survey, and 555 valid questionnaires were collected. Theoverall mean scores for career success and work environment were 3.75 [standard deviation (SD) = 1.01] and 3.42 (SD = 0.77) respectively. There was a strong positive correlation between career success and work environment (r = 0.742, p < 0.01). Multiple regression showed that attending academic conferences, psychological care, professional development, support and care, salary, and welfare were significant factors that influence career success. attending academic conferences, psychological care, and work environment are positively related to career success. Administrators should consider ways to address these factors.

Objective: To characterize and identify depressive symptoms, anxiety, and stress associated with the COVID-19 Infodemic in the elderly from São Paulo., Method: Exploratory and cross-sectional study with the elderly in the capital of São Paulo who had internet access. The sociodemographic profile, the COVID-19 infodemic, depressive symptoms, stress, and anxiety were analyzed., Results: A total of 411 older people participated in the study. There was a predominance of women (76.4%), with higher education (57.9%), using private health services, and with little income variation. Older people were more exposed to news or information about COVID-19 on the internet (45.3%), followed by television (34.5%), and radio (11.4%). The average stress was 19.96 points; 33.1% had anxiety, and 39.7% had depressive symptoms. The greater the number of people living with the elderly, the greater the stress (p = 0.001) and anxiety (p = 0.02). The hours of exposure to information on the internet led to stress (p = 0.001), depressive symptoms (p = 0.02), and anxiety (p = 0.02) in the elderly., Conclusion: During the pandemic, exposure to information on the internet triggered anxiety, stress, and depressive symptoms in the elderly. The findings highlight the need for multi and interdisciplinary interventions to mitigate such repercussions on the elderly's health.

Objective: This study aimed to investigate the impact of post-thoracotomy analgesia with dexmedetomidine and morphine on immunocytes., Methods: A total of 118 patients with post-thoracotomy Patient-Controlled Intravenous Analgesia (PCIA) in our hospital from March 2016 to July 2018 were randomly selected and divided into the Composite (COM) Group (57 patients administered with dexmedetomidine [1.0 μg.kg -1 body weight] and morphine [0.48 mg.kg -1 body weight]) and the Morphine (MOR) Group (61 patients administered with morphine [0.48 mg.kg -1 ]). The values of lymphocyte subsets (CD3+, CD4+, and CD8+) and Natural Killer cells in the peripheral blood of these two groups were detected by FACSCalibur flow cytometry at different time points (before anesthesia induction [T0], immediately after tracheal extubation [T1], 12 hours after surgery [T2], 24 hours after surgery [T3], 48 hours after surgery [T4], 72 hours after surgery [T5], and 7 days after surgery [T6]). The doses of morphine at T3 to T5 and the adverse reactions between the two groups were also recorded and compared., Results: The CD3+ level and the CD4+/CD8+ ratio at T2 to T5 and the CD4+ level and NK cells at T3 to T5 were significantly higher in the COM Group than in the MOR Group (p < 0.05). The postoperative morphine dose and the incidence of postoperative itching, nausea, and vomiting were significantly lower in the COM Group than in the MOR Group (p < 0.05)., Conclusions: Dexmedetomidine combined with morphine for post-thoracotomy PCIA can improve the function of immunocytes, reduce morphine consumption, and reduce the adverse reactions during analgesia induction., (Copyright © 2020 Sociedade Brasileira de Anestesiologia. Publicado por Elsevier Editora Ltda. All rights reserved.)

Objectives: To evaluate case series studies that quantitatively assess the effects of catheter-directed thrombolysis (CDT) and compare the efficacy of CDT and anticoagulation in patients with acute lower extremity deep vein thrombosis (DVT)., Methods: Relevant databases, including PubMed, Embase, Cochrane, Ovid MEDLINE and Scopus, were searched through January 2017. The inclusion criteria were applied to select patients with acute lower extremity DVT treated with CDT or with anticoagulation. In the case series studies, the pooled estimates of efficacy outcomes for patency rate, complete lysis, rethrombosis and post-thrombotic syndrome (PTS) were calculated across the studies. In studies comparing CDT with anticoagulation, summary odds ratios (ORs) were calculated., Results: Twenty-five articles (six comparing CDT with anticoagulation and 19 case series) including 2254 patients met the eligibility criteria. In the case series studies, the pooled results were a patency rate of 0.87 (95% CI: 0.85-0.89), complete lysis 0.58 (95% CI: 0.40-0.75), rethrombosis 0.11 (95% CI: 0.06-0.17) and PTS 0.10 (95% CI: 0.08-0.12). Six studies comparing the efficacy outcomes of CDT and anticoagulation showed that CDT was associated with a reduction of PTS (OR 0.38, 95%CI 0.26-0.55, p<0.0001) and a higher patency rate (OR 4.76, 95%CI 2.14-10.56, p<0.0001)., Conclusion: Acute lower extremity DVT patients receiving CDT were found to have a lower incidence of PTS and a higher incidence of patency rate. In our meta-analysis, CDT is shown to be an effective treatment for acute lower extremity DVT patients.

Background: The cardiac hypertrophy (CH) model for mice has been widely used, thereby providing an effective research foundation for CH exploration., Objective: To research the effects of CH modeling under abdominal aortic constriction (AAC) using different needles and weights in mice., Methods: Four needles with different external diameters (0.35, 0.40, 0.45, and 0.50 mm) were used for AAC. 150 male C57BL/6 mice were selected according to body weight (BW) and divided into 3 weight levels: 18 g, 22 g, and 26 g (n = 50 in each group). All weight levels were divided into 5 groups: a sham group (n = 10) and 4 AAC groups using 4 ligation intensities (n = 10 per group). After surgery, survival rates were recorded, echocardiography was performed, hearts were dissected and used for histological detection, and data were statistically analyzed, P < 0.05 was considered statistically significant., Results: All mice died in the following AAC groups: 18g/0.35 mm, 22 g/0.35 mm, 26 g/0.35 mm, 22 g/0.40 mm, and 26 g/0.40 mm. All mice with AAC, those ligated with a 0.50-mm needle, and those that underwent sham operation survived. Different death rates occurred in the following AAC groups: 18 g/0.40 mm, 18 g/0.45 mm, 18 g/0.50 mm, 22 g/45 mm, 22 g/0.50 mm, 26 g/0.45 mm, and 26 g/0.50 mm. The heart weight/body weight ratios (5.39 ± 0.85, 6.41 ± 0.68, 4.67 ± 0.37, 5.22 ± 0.42, 4.23 ± 0.28, 5.41 ± 0.14, and 4.02 ± 0.13) were significantly increased compared with those of the sham groups for mice with the same weight levels., Conclusion: A 0.45-mm needle led to more obvious CH than did 0.40-mm and 0.50-mm needles and caused extraordinary CH in 18-g mice.