Search

Submitted by Renorbio (renorbioba@ufba.br) on 2018-04-26T18:05:48Z No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Approved for entry into archive by Edvaldo Souza (edvaldosouza@ufba.br) on 2018-05-15T19:28:40Z (GMT) No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Made available in DSpace on 2018-05-15T19:28:40Z (GMT). No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Previous issue date: 2017-11-05 This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture.

Submitted by Renorbio (renorbioba@ufba.br) on 2018-04-26T18:05:48Z No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Approved for entry into archive by Edvaldo Souza (edvaldosouza@ufba.br) on 2018-05-15T19:28:40Z (GMT) No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Made available in DSpace on 2018-05-15T19:28:40Z (GMT). No. of bitstreams: 1 Abiotic factors influencing podophyllotoxin and yatein overproduction in.pdf: 597838 bytes, checksum: fb8a604acb73230b893d24a0d35dd3bc (MD5) Previous issue date: 2017-11-05 This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture.

Podophyllotoxin is the most studied lignan because of its use as an antimitotic agent and because it is a precursor of pharmacologically active derivatives. This review describes the anticancer activities of podophyllotoxin and the different processes that have been developed for its extraction and purification from Podophyllum spp. In addition, the synthesis routes of this compound and the development of three semi-synthetic procedures to obtain etoposide, teniposide, and Etopophos are detailed.