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The study demonstrates the importance of the antibiogram in the determination of the sensitivity of microbiocenosis of the infected dental canals. The methods and the techniques for the sampling of the pathological products are presented. The classical technique for antibiograms, consisting in horizontal diffusimetry is compared with the modern method of double-layer diffusimetry which employs a "redox" indicator (C.T.T.-triphenyl-tetrazolium-2, 3, 5-chloride) which accelerates the lecture of the results by altering the colour of the culture medium. The study was carried out in 200 patients and showed that the antibiotics with the widest spectrum on the microbiocenosis of the infected dental canals were, by order of efficiency, the following: erythromycin, chloramphenicol, ampicillin, and tetracycline. Antibiograms should be done in the shortest possible time, and the time for the lecture of the results was reduced from 36-48 hours in the case of the classical antibiograms to 16-18 hours in the case of the rapid technique. The shortening of the working time allows for a more rapid individualization of the treatment, and the preparation of antibiotic-incorporating pastes that will reduce the number of procedures to 1-2 procedures in the simple form of the pulp gangrene and to 3-4 procedures in the cases of complicated pulpal gangrene.

Anatomoclinical studies have been carried out on tissue samples obtained from patients with various chronic general diseases including cardiovascular diseases, diabetes, kidney diseases, liver diseases, acute and chronic leukemias, that had developed before the time when biologic therapy of the dental pulp had been recommended. In the presence of lesions of various severities of the dental pulp direct application of various biological techniques may trigger violent reactions, just as if the biological substances would represent deleterious factors. Vascular changes, followed by reactional responses in various stages of trophic changes, or dysplasia in the dental pulp, as demonstrated by microscopical studies show a certain degree of deterioration of the dental pulp which are counterindicative for biological therapy. When degenerative lesions of the pulpal connective structures, and vascular-nervous complex have occurred, or when leukemic invading processes have been identified biological therapy is completely counterindicated.