30 results
Search Results
2. [Changes in the immunoglobulin system in multiple myeloma].
- Author
-
Mukhadze MG, Stefani DV, and Soseliia TS
- Subjects
- Adult, Aged, Blood Protein Electrophoresis, Electrophoresis, Paper, Female, Humans, Immunodiffusion, Male, Middle Aged, Immunoglobulins analysis, Multiple Myeloma immunology
- Published
- 1974
3. [Normal immunoglobulins in different forms of monoclonal immunoglobulinopathies].
- Author
-
Andreeva NE, Fuzaĭlova LM, and German GP
- Subjects
- Electrophoresis, Paper, Humans, Immunodiffusion, Immunoelectrophoresis, Blood Protein Disorders immunology, Immunoglobulins analysis, Multiple Myeloma immunology, Waldenstrom Macroglobulinemia immunology
- Published
- 1974
4. [Effect of chronic focal infection of the maxillary sinuses on certain immunological indicators in children].
- Author
-
Tarasov DI and Lazarev VN
- Subjects
- Adolescent, Blood Proteins analysis, Child, Chronic Disease, Electrophoresis, Paper, Humans, Immunodiffusion, Immunoglobulin A isolation & purification, Immunoglobulin G isolation & purification, Immunoglobulin M isolation & purification, Immunoglobulins isolation & purification, Maxillary Sinus immunology, Sinusitis immunology
- Published
- 1974
5. [The issue of use parallelism factor method and index of circulating anticoagulant in diagnostic algorithm deficity of coagulation factors.]
- Author
-
Potylitsina VV, Samoilenko VV, Lobanova SM, Olkhovskiy IA, and Palgova IV
- Subjects
- Adult, Algorithms, Child, Humans, Blood Coagulation Factors analysis, Hemophilia A diagnosis, Immunoglobulins blood
- Abstract
The paper presents the comparative use of algorithms to identify the causes of deficiency of coagulation factors in patients with a prolonged APTT, including the definition of the index of circulating anticoagulant (ICA) and the factor-parallelism (FP) method. The results obtained in children with hereditary hemophilia and adults with acquired hemophilia. It is shown that ICA is an effective method for pre-selection of patients with hereditary hemophilia if you suspect an inhibitor to subsequent confirmation test Bethesda. The method the FP has just proved itself in the diagnosis of acquired forms of hemophilia. The use of FP method is most expedient at the stage of screening to identify inhibitors in the laboratory. Method FP loses diagnostic value if the results of the activity factor in all dilutions is close to zero, which is characteristic for individual variants of hereditary hemophilia., Competing Interests: The authors declare no conflict of interest.
- Published
- 2019
- Full Text
- View/download PDF
6. [Regulatory role of the immune system in the organism].
- Author
-
Alekseev LP and Khaitov RM
- Subjects
- Aggression physiology, Biological Evolution, Cell Communication immunology, Cytokines immunology, HLA Antigens chemistry, HLA Antigens classification, Humans, Immune System cytology, Immunoglobulins chemistry, Lymphocytes cytology, Lymphocytes immunology, Models, Molecular, HLA Antigens immunology, Immune System physiology, Immunoglobulins physiology, Reproduction immunology
- Abstract
The paper presents modern idea of regulatory role of the human immune system in performing a number of physiological functions including intercellular interactions, reproductive process, and forming of protection against external and internal aggression. Significance of the immune system is considered and substantiated, that of genes of the human immune response in particular in provision of human survival as a biological species.
- Published
- 2010
7. [Chicken yolk IgY preparations as immune reagents to tick-borne encephalitis virus].
- Author
-
Ivanov AP and Vargin VV
- Subjects
- Animals, Antibody Specificity, Antigens, Viral analysis, Antigens, Viral immunology, Encephalitis Viruses, Tick-Borne isolation & purification, Encephalitis, Tick-Borne diagnosis, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulins isolation & purification, Chickens immunology, Egg Yolk immunology, Encephalitis Viruses, Tick-Borne immunology, Immunoglobulins immunology
- Abstract
This paper provides the first (as is known from the literature analysis) results of the use of specific egg yolk IgY from immunized hens to prepare immune reagents to tick-borne encephalitis virus (TBEV). The IgY preparations are shown to possess a high specific activity in ELISA and can be used as reference reagents, and also for the construction of ELISA systems to detect TBEV antigen.
- Published
- 2010
8. [Efficiency of use of immunoglobulin preparations for the postexposure prevention of tick-borne encephalitis in Russia (a review of semi-centennial experience)].
- Author
-
Pen'evskaia NA and Rudakov NV
- Subjects
- Animals, Humans, Immunization Schedule, Immunoglobulins administration & dosage, Russia, Encephalitis Viruses, Tick-Borne immunology, Encephalitis, Tick-Borne prevention & control, Immunization, Passive, Immunoglobulins therapeutic use, Post-Exposure Prophylaxis
- Abstract
Along with regenerated interest in passive immunization during flavivirus infections, there is an increase in the number of reports with negative opinions as to the expediency of postexposure immunoglobulin prophylaxis (IGP) of tick-borne encephalitis (TBE). Analysis of the publications (since 1959) on this problem has indicated that more than half of them, including all papers with negative opinions as to IGP, contain the results of studies whose design are unsuitable for the solution of the problem associated with the efficiency of medical interventions in the context of evidence-based medicine. Analysis of the results of cohort studies has established that the timely single administration of an immunoglobulin (IG) preparation against TBE in a dose of 0.05 ml/kg body weight ensures protection on average in 79% of cases. Increasing the single dose to 0.1 ml/kg, as re-administration of IG, provides no additional protective effect. The preventive efficacy of IG preparations against TBE varies under the influence of factors, such as the infectious virus dose inoculated from tick suckling, the age of a victim, the antiviral activity of preparations with a antihemagglutinin titer of 1:20 with high infectious virus doses and the absence of reliable rapid tools for its determination on tick suckling, it is necessary to use IG with a titer of at least 1:80 and, desirably, more active in children, for postexposure prophylaxis of TBE.
- Published
- 2010
9. [Current approaches to emergency specific prevention of tick-borne encephalitis].
- Author
-
Kozlova IV, Zlobin VI, Verkhozina MM, Demina TV, Dzhioev IuP, Lisak OV, Doroshchenko EK, Khasnatinov MA, Danchinova GA, and Adel'shin RV
- Subjects
- Animals, Bites and Stings, Costs and Cost Analysis, Diagnosis, Differential, Encephalitis, Tick-Borne diagnosis, Humans, Injections, Intramuscular, Ixodes virology, Encephalitis Viruses, Tick-Borne immunology, Encephalitis, Tick-Borne prevention & control, Immunization, Passive economics, Immunoglobulins administration & dosage
- Abstract
Emergency specific prevention of tick-borne encephalitis (TBE) by using homologous immunoglobulin is an important element in the package of controlling measures against this viral natural and focal infection. There are annually a few hundred thousand referrals for health care facilities for tick bites. Their maximum coverage tactics via immunoglobulin prevention is medically unreal and unjustifiable. The paper presents the results of a long-term application of another approach based on preliminary rapid studies of the ticks taken from victims or the blood of patients in the period of possible development of virusemia and preventive immunoglobulin use only in the persons bitten with TBE virus-infected ticks. Examination of the material available from more than 56 thousand referrals indicated the high epidemiological (more than 99%) and economic effectiveness of the target administration of an immunological drug. By taking into account the accumulated data on a wide spread of combined foci of TBE and other tick-borne infections and the authors' own experience, it is suggested that it is necessary to organize a comprehensive differential laboratory diagnosis and emergency prevention against the whole complex of Ixodes tick-borne infections.
- Published
- 2007
10. [Detection of rearranged immunoglobulin and T-cell receptor genes as a method for characterization of tumor cell clonality in acute lymphoblast leukemia in children].
- Author
-
Meleshko AN and Potapnev MP
- Subjects
- Adolescent, Bone Marrow Cells immunology, Burkitt Lymphoma diagnosis, Burkitt Lymphoma genetics, Burkitt Lymphoma immunology, Child, Child, Preschool, Humans, Leukemia-Lymphoma, Adult T-Cell diagnosis, Leukemia-Lymphoma, Adult T-Cell genetics, Leukemia-Lymphoma, Adult T-Cell immunology, Precursor Cell Lymphoblastic Leukemia-Lymphoma immunology, Gene Rearrangement, Immunoglobulins genetics, Precursor Cell Lymphoblastic Leukemia-Lymphoma diagnosis, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics, Receptors, Antigen, T-Cell genetics
- Abstract
Selection of primers for polymerase chain reaction (PCR) as used in detection of the reconstructed genes of immunoglobulins and T-cells receptor in tumor cells in children with acute lymphatic leukemia is described in the paper. PCR potentialities and limitations in characterizing the monoclonality observed in primary acute lymphatic leukemia are demonstrated. Cross linkages of the heavy chain of immunoglobulins and of T-cell receptor were found between T- and B-linear acute lymphatic leukemia forms, but not between acute lymphatic leukemia forms and myeloleukemia in children.
- Published
- 2004
11. [Complement-binding and immuno-modulating properties of polyreactive immunoglobulins].
- Author
-
Bobrovnik SA, Efetov KA, Petrova IuI, and Komisarenko SV
- Subjects
- Animals, Antibodies, Monoclonal immunology, Antigen-Antibody Reactions, Antigens immunology, Binding, Competitive, Complement C1q metabolism, Female, Immunization, Passive, Immunoglobulins metabolism, Injections, Intravenous, Male, Mice, Mice, Inbred CBA, Adjuvants, Immunologic metabolism, Antibody Formation immunology, Complement C1q immunology, Immunoglobulins immunology
- Abstract
New data concerning biological properties of polyreactive immunoglobulins (PRIG) were obtained as a result of treatment of mouse serum immunoglobulins by 4 M KSCN and are presented in the paper. In particular, the capacity of PRIG to bind C1q, the subunit of the first component of complement was studied. It was shown that PRIG's binding capacity to C1q is similar to that of intact immunoglobulins. Intravenous administration of PRIG into mice together with either sheep red blood cells or heat-inactivated staphylococcal bacteria did not affect the immune response to these antigens. Meanwhile, the same administration of PRIG together with the purified protein derivate of tuberculin resulted in 10-fold increase of mouse antibody response to PPD. These results demonstrate that PRIG can have some immuno-modulating properties concerning low-immunogenic antigens.
- Published
- 2003
12. [Specific immunoglobulins as effective drugs for treatment of virus infections: present state and urgent tasks in Ukraine].
- Author
-
Diachenko NS, Kurishchuk KV, Riadskaia LS, Netreba NI, Pisareva SP, Mikhaĭlova EG, Nesterova NV, Zagorodniaia SD, Spivak NIa, Lozitskiĭ VP, and Smirnov VV
- Subjects
- Biotechnology, Herpesviridae Infections therapy, Humans, Ukraine, Immunoglobulins therapeutic use, Infections therapy, Virus Diseases therapy
- Abstract
The thesis about importance of specific immunoglobulin preparations in treatment of infectious diseases of viral and microbial etiology, uncontrollable infections, caused by representatives of herpes viruses family in particular, is developed in the paper. The types of immunoglobulin preparations, their advantages, technology peculiarities has been considered; the experience of application, including that of the authors have been analyzed. The necessity and expedience of intensive development of this branch of immunobiotechnology in Ukraine is proved, especially in connection with a high level of infectious morbidity in this country, concrete recommendations are given.
- Published
- 2002
13. [The outlook for improving the immunoglobulin preparations for the detection and identification of the causative agents of glanders and melioidosis].
- Author
-
Khrapova NP, Tikhonov NG, Prokhvatilova EV, and Kulakov MIa
- Subjects
- Agglutination Tests methods, Agglutination Tests standards, Agglutination Tests statistics & numerical data, Animals, Burkholderia pseudomallei immunology, Hybridomas immunology, Immunodiffusion methods, Immunodiffusion standards, Immunodiffusion statistics & numerical data, Indicators and Reagents standards, Mice, Mice, Inbred BALB C, Pseudomonas immunology, Antibodies, Monoclonal isolation & purification, Burkholderia pseudomallei isolation & purification, Immunoglobulins isolation & purification, Pseudomonas isolation & purification
- Abstract
The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.
- Published
- 1995
14. [Clinical and prognostic significance of cellular immunity factors in patients with chronic liver diseases].
- Author
-
Podymova SD and Rachvelishvili NB
- Subjects
- Adolescent, Adult, Aged, Antibodies, Monoclonal, Biomarkers blood, CD4-CD8 Ratio, Female, Humans, Immunocompetence, Male, Middle Aged, Phenotype, Prognosis, Rosette Formation, Hepatitis B immunology, Hepatitis B Surface Antigens blood, Hepatitis, Chronic immunology, Immunoglobulins blood, Liver Cirrhosis, Alcoholic immunology, T-Lymphocytes immunology
- Abstract
The paper presents the results of comprehensive studies of quantitative and functional parameters of cellular immunity in 283 patients with chronic liver diseases, using various rosette tests and monoclonal antibody phenotyping of immunocompetent cells. The pattern of changes in blood immunocompetent cells and immunoglobulins allows prediction of the natural history of chronic active hepatitis and hepatic cirrhosis of viral and alcoholic origin. The data on autorosette-forming and hepatic-specific lipoprotein-sensitive rosette-forming cells were found to be the most informative. As compared to health, there were substantial changes in the subpopulation composition of immunocompetent cells and immunoglobulins in hepatic cirrhosis. The changes in immunoregulatory theophylline-resistant (OKT4+, OKT8+, T mu and T gamma) and theophylline-sensitive E-rosetting, as well as effector (ICO11+ and ICOHM1+) cells were the leading immunopathogenetic link of hepatic cirrhosis. In the latter, the subpopulation composition of immunocompetent cells depended on the activity of a pathological process, the state of compensation, as well as the levels of HBsAg and blood anti-delta-antibodies. The subpopulation analysis of immunocompetent cells enabled the author to outline the specific features of the immune status of etiological types, as well as predictive types of chronic active hepatitis and hepatic cirrhosis, which may serve as evidence for goal-oriented immunomodulating therapy.
- Published
- 1994
15. [Empirical construction of the evolutionary divergence tree of VH-genes of immunoglobulins in mouse family 36-60].
- Author
-
Urakov DN and Deev SM
- Subjects
- Animals, Base Sequence, Codon genetics, Mice, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Immunoglobulins genetics, Phylogeny
- Abstract
Formerly unknown variable gene of the heavy chain of immunoglobulin (VHTF) was studied in our preceding works in detail. In this paper, we present the results of defining the structure of N-terminus of the heavy chain which confirm the presence of unusual first codon corresponding to threonine. The structure of the VHTF gene is being compared with that of other genes of the 36-60 family, topology of the tree of their evolutionary divergence is being established with the help of empirical algorithm. Its advantages, as compared with the conventional methods, are under discussion.
- Published
- 1991
16. [Use of the method of immunothermistography in obstetrical practice].
- Author
-
Paliĭ GK and Berezovskaia SB
- Subjects
- Antigen-Antibody Reactions immunology, Antigens, Bacterial immunology, Female, Hot Temperature, Humans, Immunologic Techniques, Pregnancy, Pregnancy Complications, Infectious diagnosis, Pregnancy Complications, Infectious immunology, Puerperal Infection diagnosis, Puerperal Infection immunology, Staphylococcal Infections diagnosis, Staphylococcal Infections immunology, Staphylococcal Toxoid immunology, Hemolysin Proteins analysis, Immunoglobulins, Pregnancy Complications, Infectious prevention & control, Puerperal Infection prevention & control, Staphylococcal Infections prevention & control, Staphylococcal Toxoid administration & dosage, Staphylococcus immunology
- Abstract
This paper presents results of testing pregnant women for staphylococcal antigen sensitization using immunothermistography (ITG). The ITG is based on registration of environmental heat conduction change with a microthermistor resistor during antigen-antibody reaction. The study group comprised 75 pregnant women immunized by staphylococcal anaphylotoxin and nonimmunized pregnant and nonpregnant women. Blood alfa-antistaphylolysin levels showed a close direct correlation with ITG findings. Combined use of these methods identified a population of pregnant women requiring immunization.
- Published
- 1990
17. [Homeostatic effect of thymalin in multiple sclerosis].
- Author
-
Akimov GA, Golovkin VI, and Khavinson VKh
- Subjects
- Adrenal Cortex Function Tests, Adrenal Glands drug effects, Adrenocorticotropic Hormone blood, Adult, Female, Homeostasis drug effects, Homeostasis physiology, Humans, Hydrocortisone blood, Immunoglobulins drug effects, Male, Middle Aged, Multiple Sclerosis physiopathology, Pituitary Function Tests, Pituitary Gland, Anterior drug effects, Rosette Formation, T-Lymphocytes drug effects, Adrenal Glands physiopathology, Immunoglobulins analysis, Multiple Sclerosis drug therapy, Pituitary Gland, Anterior physiopathology, T-Lymphocytes immunology, Thymus Hormones therapeutic use
- Abstract
The paper is concerned with interaction of the immune, endocrine and nervous systems in patients with multiple sclerosis. Dexamethasone and vasopressine tests were made. Lymphocyte sensitivity to hydrocortisone and thymalin, the main protein of myelin, was detected. It is advisable that arginine-vasopressine combined with thymalin be used for correction of homeostatic abnormalities and secondary disease prophylaxis.
- Published
- 1990
18. [Specific immunoglobulins of different classes in brucellosis in human beings].
- Author
-
Zheludkov MM, Chernysheva MI, Pavlova IP, Suleĭmanov AK, Kurmanova KB, Pol'shchikova NN, and Perekopskiĭ IS
- Subjects
- Acute Disease, Bacterial Vaccines immunology, Chronic Disease, Cross Reactions immunology, Francisella tularensis immunology, Humans, Immunoenzyme Techniques, Immunoglobulin A analysis, Immunoglobulin G analysis, Immunoglobulin M analysis, Antibody Specificity immunology, Brucella abortus, Brucellosis immunology, Immunoglobulins analysis
- Abstract
The paper is concerned with the results of studying the level of specific IgG, IgA and IgM in people with different forms of brucellosis. Immunoglobulins belonging to different classes were identified by means of IEA. Examination of 514 patients with different forms of brucellosis and 122 controls demonstrated the high specificity and sensitivity of IEA for detecting antibodies of different physiochemical nature. The use of IEA made it possible to establish definite regularities of the synthesis of specific IgG, IgA, IgM in people with brucellosis, which can be used for assessing the activity of the infectious process.
- Published
- 1990
19. Quality Control of Colostrum and Protein Calf Milk Replacers
- Author
-
Vladimir D. Kharitonov, Vladimir A. Asafov, Evgeniya L. Iskakova, Nina L. Tankova, Tatsiana M. Halavach, and Vladimir P. Kurchenko
- Subjects
colostrum ,whole milk replacers ,hydrolysis ,antigenicity ,immunoglobulins ,enzymes ,fatty acid composition ,Food processing and manufacture ,TP368-456 - Abstract
Introduction. Improving technologies and providing young farm animals with high-quality feed are the primary tasks for successful reproduction and maintenance of dairy cattle. The research objective was to assess the quality characteristics of colostrum and milk replacers, as well as their technological prospects. Study objects and methods. The research featured colostrum, calf milk replacers (CMR), processing methods, and quality characteristics. The paper introduces an analysis of various sustainable processes of obtaining new CMRs. Results and discussion. The article describes colostrum: recommended intake for young calves, qualitative characteristics, and control methods. It focuses mostly on the microbiological characteristics of colostrum, as well as on its role in developing the immune system of calves and the prospects of enzymatic regulation of its functional properties. Enzymatic regulation is based on deep proteins hydrolysates and a highly active serine protease (alcalase). The authors studied variants of using various enzyme preparations and bacterial starter cultures for obtaining hydrolyzed and fermented colostrum, analyzed the main process indicators of milk replacers with intermediate moisture content, and tested various methods for assessing the fatty acid and protein composition of concentrated milk replacers. Production methods proved to have a significant impact on the indicators in question. Conclusion. Reproduction of the dairy herd genetic potential depends on the diet of the young farm animals, and so does the economy of agricultural production. Enzymatic processing of raw materials proved to be the most promising approach for obtaining products with improved functional properties. Deep colostrum hydrolysates can also be an important part of functional foods for children, athletes, in dietary foods, etc.
- Published
- 2021
- Full Text
- View/download PDF
20. [State of the T- and B-system of immunity in normal cerebrospinal fluid from persons of different age groups].
- Author
-
Malashkiia IuA, Ratiani LN, Cheĭshvili LD, Dugladze NA, and Geladze MG
- Subjects
- Adolescent, Adult, Cell Count, Cerebrospinal Fluid cytology, Humans, Immunoglobulin A cerebrospinal fluid, Immunoglobulin G cerebrospinal fluid, Immunoglobulin M cerebrospinal fluid, B-Lymphocytes, Cerebrospinal Fluid immunology, Cerebrospinal Fluid Proteins analysis, Immunoglobulins cerebrospinal fluid, T-Lymphocytes
- Abstract
The paper is concerned with studies of the T- and B-immunity systems in the CSF in 37 patients, in whom the CSF was extracted in order to perform spinal anesthesia. This was necessary for surgical operations because of cryptochidism (16 patients aged from 16--30) and hypertrophy of the prostate (21 patients aged from 60 and over). The patients of both groups showed no signs of nervous system lesions. A comprehensive immunomorphological analysis of the CSF cells permitted to detect the medium indices of the T- and B-immunocompetent cells of individuals depneding upon the age.
- Published
- 1978
21. [Immunologic aspects of migraine headaches and autonomic prosopalgias].
- Author
-
Puzin MN, Kulakov AV, and Balashov KE
- Subjects
- Adult, Dysgammaglobulinemia etiology, Female, Humans, Hypergammaglobulinemia etiology, IgA Deficiency, Immunoglobulin A analysis, Male, Middle Aged, Saliva immunology, Facial Neuralgia immunology, Immunoglobulins analysis, Migraine Disorders immunology, Trigeminal Neuralgia immunology
- Abstract
The paper presents the results of an immunological screening of patients with facial vegetative neuralgia, Horton's syndrome, and migraines prior to and following therapy. The patients with migraines were found to have an activated local humoral immunity. Those from all the groups showed lower serum immunoglobulin A concentrations after therapeutic interventions.
- Published
- 1989
22. HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
- Author
-
M. V. Volkova, A. V. Kunder, I. I. Generalov, and D. M. Petrovich
- Subjects
immunoglobulins ,abzymes ,nuclease activity ,heterogeneity ,rheumatoid arthritis ,reactive arthritis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Catalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the nature of nuclease activity in these diseases. Determination of DNAse activity of immunoglobulins with different DNA substrates, and search for specific substrates for distinct clinical entities could serve these purposes. The aim of present work is to determine DNase activity of the polyclonal class G immunoglobulins in rheumatoid and reactive arthritis using various methods.Different methods are used to evaluate nuclease activity. In this paper we present newly developed and modified techniques for determination of DNAse activity of polyclonal IgGs. Particular attention was paid to the electrophoretic method of DNase activity assessment. Polyclonal IgG isolated from blood serum of patients with rheumatoid arthritis and reactive arthritis were used for assays. In this study, we demonstrated the presence of an inhomogeneous DNase activity of immunoglobulins in relation to different substrates.Along with calf thymus DNA, we used bacterial plasmid DNA and PCR products based on bacterial gene sequences. Levels of DNase activity by rivanol clot method with calf thymus DNA as substrate proved to be higher in patients with rheumatoid arthritis than the control values (p < 0.01). DNase abzyme activity in patients with rheumatoid arthritis was elevated, as compared to the patients with reactive arthritis (p < 0.01).When examining ability of the IgG to hydrolyze procaryotic DNA (bacterial plasmid DNA and PCR products, based on bacterial genes), we obtained heterogeneous results. Different Ig samples showed varying degrees of DNA hydrolysis. Abzyme hydrolysis of DNA substrates longer than 700 bp was more pronounced, as compared to short DNA substrates (100 base pairs).Conclusions: 1) antibodies having deoxyribonucleic activity are present in serum of patients with rheumatoid arthritis, reactive arthritis; 2)DNase abzymes exhibit heterogeneous ability to hydrolyze various DNA substrates; 3) Abzyme-medicted hydrolysis of DNA substrates longer than 700 base pairs is more pronounced, as compared to a short DNA substrates (100 bp) length.
- Published
- 2017
- Full Text
- View/download PDF
23. FEATURES OF PLASMAPHERESIS IN THE TREATMENT OF GRAFT REJECTION AFTER KIDNEY TRANSPLANTATION
- Author
-
A. V. Vatazin, A. B. Zulkarnaev, R. O. Kantarija, M. Krstich, and A. P. Faenko
- Subjects
cadaveric kidney allotransplantation ,antibodies ,immunoglobulins ,plasmapheresis ,cascade plasmapheresis ,rebound effect ,Surgery ,RD1-811 - Abstract
Introduction. The development of immunological confl ict in the form of host-versus-graft reaction has always been main problem in transplantation. The worst case is the development of humoral rejection with the presence of circulating immune complexes and antibodies. There are several methods for quick removal of antibodies; among those are traditional plasmapheresis (PA) and double fi ltration plasmapheresis (DFPF). In this paper we present our experience with these two methods and give a comparative evaluation of the effectiveness in the treatment of acute humoral rejection in renal allograft. Aim: to compare the effectiveness of traditional and double fi ltration plasmapheresis while processing different volumes of plasma in the treatment of host-versus-graft disease after kidney transplantation.Methods. The study included 58 patients after kidney transplantation. All patients had increased activity of humoral immunity, which was confi rmed by immunofl uorescence with luminescence C4d complement component. In 26 patients we performed DFPF, in 32 patients – traditional PA. We divided the DFPF patients into 4 subgroups depending on the amount of processed plasma: > 50% (5 patients), 50–100% (8 patients), 100–150% (7 patients), 150–200% (6 patients) of circulating plasma volume. We also divided PA patients into four subgroups depending on the volume of plasma removed: >50% (8 patients), 50–70% (12 patients), 70–90% (7 patients), 90–110% (5 patients) of the volume of circulating plasma. We monitored the immune status with markers of humoral immunity activation IgM, IgG before and after each of the procedures.Results. Each procedure of traditional PA and DFPF was accompanied by a marked decrease in blood concentrations of IgM and IgG antibodies. Their level decreased by an average of 30–55% of the original. However, some patients in both groups showed an increase in the concentration of these immunoglobulins in 1–2 days after the fi rst and the second procedures. The effect of rebound was observed during DFPF if in one procedure less than 100% of the circulation plasma volume was processed and during traditional PA if less than 70% was removed. Upon reaching the target values and increasing the processing volumes we managed to avoid growth of IgM and IgG.Conclusions. DFPF and traditional PA can effectively reduce the high titers of circulating antibodies, which is accompanied by a decrease in the activity of humoral immunity of the recipient. In the case of double fi ltration plasmapheresis at least one volume of circulating plasma should be processed and in traditional plasmapheresis – at least 70% should be removed.
- Published
- 2015
- Full Text
- View/download PDF
24. Quality Control of Colostrum and Protein Calf Milk Replacers
- Author
-
Tatsiana N. Halavach, Vladimir P. Kurchenko, Evgeniya Iskakova, Vladimir A. Asafov, Vladimir Kharitonov, and Nina Tankova
- Subjects
Antigenicity ,Economics, Econometrics and Finance (miscellaneous) ,enzymes ,immunoglobulins ,Industrial and Manufacturing Engineering ,03 medical and health sciences ,Hydrolysis ,fatty acid composition ,Food science ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,lcsh:TP368-456 ,Chemistry ,0402 animal and dairy science ,food and beverages ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,lcsh:Food processing and manufacture ,Enzyme ,whole milk replacers ,colostrum ,hydrolysis ,antigenicity ,Colostrum ,Fatty acid composition ,Food Science - Abstract
Introduction. Improving technologies and providing young farm animals with high-quality feed are the primary tasks for successful reproduction and maintenance of dairy cattle. The research objective was to assess the quality characteristics of colostrum and milk replacers, as well as their technological prospects. Study objects and methods. The research featured colostrum, calf milk replacers (CMR), processing methods, and quality characteristics. The paper introduces an analysis of various sustainable processes of obtaining new CMRs. Results and discussion. The article describes colostrum: recommended intake for young calves, qualitative characteristics, and control methods. It focuses mostly on the microbiological characteristics of colostrum, as well as on its role in developing the immune system of calves and the prospects of enzymatic regulation of its functional properties. Enzymatic regulation is based on deep proteins hydrolysates and a highly active serine protease (alcalase). The authors studied variants of using various enzyme preparations and bacterial starter cultures for obtaining hydrolyzed and fermented colostrum, analyzed the main process indicators of milk replacers with intermediate moisture content, and tested various methods for assessing the fatty acid and protein composition of concentrated milk replacers. Production methods proved to have a significant impact on the indicators in question. Conclusion. Reproduction of the dairy herd genetic potential depends on the diet of the young farm animals, and so does the economy of agricultural production. Enzymatic processing of raw materials proved to be the most promising approach for obtaining products with improved functional properties. Deep colostrum hydrolysates can also be an important part of functional foods for children, athletes, in dietary foods, etc.
- Published
- 2021
25. Common variable hypogammaglobulinemia: The author's observation
- Author
-
A I Parfenov, L M Krums, O V Akhmadullina, P L Shcherbakov, S G Khomeriki, N V Orlova, E A Sabel'nikova, and N A Fadeeva
- Subjects
immunoglobulins ,common variable hypogammaglobulinemia ,nodular lymphoid hyperplasia ,plasma cells ,excessive bacterial growth in the small intestine ,Medicine - Abstract
The paper gives a case history of a patient with common variable hypogammaglobulinemia (СVHGG). Its clinical picture was characterized by frequent inflammatory airway diseases, pansinusitis, and periodic rises in temperature up to febrile ones. The diagnosis was established on the basis of the decreased serum concentrations of immunoglobulins (IG) A, M, and G, the unusual small intestinal (SI) wall relief created by large lymphoid follicles, as well as the characteristic sign of CVHGG, namely, the lack of plasma cells in the lamina propria of the SI mucosa. Antibacterial therapy was noted to have a good effect that confirmed the leading role of excessive bacterial growth in the pathogenesis of chronic diarrhea and fever, which are the major clinical manifestations of CVHGG in this patient.
- Published
- 2015
26. CLINICAL MANIFESTATIONS OF VASCULITIS AT THE ONSET OF MULTIPLE MYELOMA
- Author
-
V I Vasil'ev, V R Gorodetskiy, S Kh Sedyshev, N A Probatova, A I Pavlovskaya, M Yu Varlamova, S G Pal'shina, A L Logunov, O A Logvinenko, E V Sokol, E L Nasonov, V I Vasilyev, V R Gorodetsky, S G Palshina, and O Logvinenko
- Subjects
myeloma ,vasculitis ,immunoglobulins ,cryoglobulins ,sepsis ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
The paper describes two patients (a 50-year woman and a 72-year man) in whom the clinical manifestations of ulceronecrotic vasculitis had long preceded before the diagnosis of multiple myeloma was made. In the former, monoclonal cryoglobulinemia type I induced ulcerative lesions with the development of dry toe gangrene and paraproteinemic renal lesion. In the latter, ulceronecrotic vasculitis with the development of dry toe gangrene was a manifestation of monoclonal paraproteinemia without signs of cryoglobulinemia. Both patients were found to have monoclonal blood secretion (РIgGλ и РIgGκ) and urine Bence Jones protein (BJλ+BJκ, BJκ) in the absence of immunological markers of vasculitis developing in patients with rheumatic diseases. Immunochemical study of serum/urine and, when monoclonal secretion of PIg+BJ is detected, further examination for plasma cell dyscrasia should be performed in all cases of vascular disorders (cold allergy, Raynaud's syndrome, purpura, ulcers of cruses, and gangrene of distal phalanxes of the hands/feet) in patients with atypical vasculitis. The timely immunochemical study of blood and urine will make it possible to diagnose plasma cell dyscrasia (different types of myelomic disease, Waldenstrцm macroglobulinemia, primary amyloidosis) at the early stage of the disease and to rule out unjustifiably diagnosed vasculitis in patients with atypical vascular lesions.
- Published
- 2010
- Full Text
- View/download PDF
27. COMPARATIVE CHARACTERISTICS OF THE IMMUNOGLOBULINS OF BREAST MILK AND BLOOD SERUM IN THE POSTNATAL PERIOD IN WOMEN WITH DIFFERENT HEALTH LEVELS
- Author
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Yu. N. Dementeva, A. P. Cherdantsev, A. I. Kuselman, A. A. Guschina, and I. R. Bahtogarimov
- Subjects
biology ,business.industry ,breastfeeding ,Breastfeeding ,immunoglobulins ,Physiology ,food and beverages ,Breast milk ,Immunoglobulin E ,immunity ,Pediatrics ,Peripheral blood ,RJ1-570 ,medicine.anatomical_structure ,fluids and secretions ,children ,Immunity ,Lactation ,Pediatrics, Perinatology and Child Health ,biology.protein ,Medicine ,Antibody ,business - Abstract
Breast milk is considered to be the best nutrition for infants, as it provides all the nutrients necessary for proper development during the first 6 months of life. Objectives: to study the level of immunoglobulins of the main classes in breast milk and peripheral blood in women with different health status in lactation dynamics. The IgA, sIgA, IgE, IgM, IgG parameters in breast milk and peripheral blood were determined in 3-5 days after delivery and in 1, 3 and 6 months of lactation.Results.The paper presents data on the level of immunoglobulins in 96 women with different health status. It demonstrates that female milk contains the main classes of immunoglobulins which have reverse dynamics of secretion dependent on the lactation term.
- Published
- 2018
28. Relation between Helicobacter pylori Infection with Thyroid Autoantibodies in Dyspeptic Patients.
- Author
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Antikchi, Mohammad Hossein, Mirhosseini, Hamid, Salami, Maryam Sadat, Bidaki, Reza, Hosseini, Omid Reza, Abasi, Ali Reza Molla, and Bashardoost, Nasroola
- Subjects
HELICOBACTER pylori infections ,HELICOBACTER pylori ,PROTEINS ,IMMUNOGLOBULINS ,GASTROESOPHAGEAL reflux - Abstract
Background : Infections attributed to Helicobacter pylori (H. pylori) involve almost half of the world's population. One of the effects is auto-antibody induction and cross-reaction with numerous proteins in the body. As a result of its widespread prevalence and importance, this study evaluates the associations between H. pylori and thyroid auto-antibodies. Materials and Methods: This study enrolled 100 patients who were candidates for gastroesophageal endoscopy that referred to the Yazd Gastrointestinal Clinic. Patients underwent the following laboratory analyses: urease test, anti-H. pylori (IgG), TSH, T4, T3 and thyroid auto-antibodies (anti-thyroglobulin, anti-thyroid peroxides). Patients were divided into two groups of H. pylori positive and negative according to the results of the anti-H. pylori IgG and urease tests. The level of thyroid auto-antibodies and thyroid function tests were compared between groups. Data were analyzed with chi-square and t-tests. SPSS software version 17 was used for data analysis. Results: Overall, 61% of patients were H. pylori positive. The mean anti-Thyroid peroxidase level in the H. pylori positive group was significantly more than the negative group (p<0.01). In addition, 19.7% of H. pylori positive patients and 5.1% of H. pylori negative patients had positive anti-TPO levels, which the difference between both groups was significant (p<0.04). There was no significant difference in thyroid function between the two groups. Conclusion: Although no significant difference in thyroid function was seen in the two groups more patients tested positive for anti-TPO levels in the H. pylori positive group, which was suggestive of thyroid auto-antibody induction by H. pylori. [ABSTRACT FROM AUTHOR]
- Published
- 2012
29. Immunological aspects of breastfeeding
- Author
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Yu. N. Dementyeva
- Subjects
fluids and secretions ,breastfeeding ,immunoglobulins ,bacteria ,food and beverages ,babies ,biochemical phenomena, metabolism, and nutrition ,skin and connective tissue diseases ,immunity ,Pediatrics ,cytokines ,RJ1-570 ,lactoferrin - Abstract
The paper reviews the literature on the impact of different breast milk parameters on a baby's immunity. It characterizes humoral and cellular factors and other protective components in breast milk. There is evidence for the advantage of breastfeeding for the development of the immune system in infants.
- Published
- 2016
30. ИЗМЕНИЕ ПОКАЗАТЕЛЕЙ РОТОВОЙ ЖИДКОСТИ У ЖЕНЩИН РЕПРОДУКТИВНОГО ВОЗРАСТА В РАЗНЫХ ФАЗАХ МЕНСТРУАЛЬНОГО ЦИКЛА
- Subjects
гонадотропные и стероидные гормоны ,ротовая жидкость ,immunoglobulins ,иммуноглобулины ,oral fluid ,gonadotropic and steroid hormones ,menstrual cycle ,менструальный цикл - Abstract
В статье представлены результаты исследования гонадотропных и стероидных гормонов, иммунологические показатели ротовой жидкости, а также некоторые физико-химические свойства слюны в разных фазах менструального цикла у женщин репродуктивного возраста. Выявлена их зависимость от фазы менструального цикла. Полученные данные позволяют правильно интерпретировать изменения этих показателей при различных заболеваниях зубо-челюстной системы, оценить эффективность проведенного лечения и выбрать оптимальные сроки планирования хирургических вмешательств в тканях полости рта и челюстно-лицевой области., The paper presents the results of the study of gonadotropic and steroid hormones, immunological parameters of the oral fluid, as well as some physical and chemical properties of saliva at different phases of the menstrual cycle in women of reproductive age. It was revealed the dependence of these parameters on the phase of the menstrual cycle. The obtained data allow correctly interpret the changes in these parameters for various diseases of the dentition, evaluate the effectiveness of the treatment and select the optimal timing for planning surgical interventions in the tissues of the oral cavity and maxillofacial area.
- Published
- 2017
- Full Text
- View/download PDF
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