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DNA of H-1, H-5, H-10, H-17, and H-22 phages were studied, their nucleotide composition, their buoyant densities in cesium chloride and cesium sulphate, curves of melting and spectra of circular dichroism were determined 5-oxymethylcytosine in the composition of H-I and H-22 phage DNA was shown immunologically to be glucozylated.

A comparative study of the reactivities of free 5-hydroxymethylcytosine (5-HMC) and 5-HMC found in the composition of native, denaturated and intraphage DNA of the T2 phage with that of O-methylhydroxylamine (OMHA) demonstrated that the DNA secondary structure in situ is partially disturbed. The interaction DNA-protein in the phage particle channels the reaction into a predominant formation of 4N-methoxy-6-methoxyamino-5,6-dihydro-5-hydroxymethyl cytosine, but not 4N-methoxy-5-hydroxymethyl cytosine, which is formed in vitro. In the course of the reaction the interaction DNA-protein is probably fixed by covalent binding.