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The solvent system of alcohol-acid-water gradually forms an equilibrated amount of ester which facilitates the separation of di- and tri-carboxylic acids on paper chromatograms. Using the system of n-butanol-formic acid-water (3:1:1) as an illustration, two methods of accelerating the system ageing are demonstrated: 1) butanol-acid mixture at a required ratio is kept for several hours before water is added and 2) butanol-acid mixture is passed through the KU-2 layer in the H-form. The effect identical to that achieved as a result of 5-day storage of the solvent system under usual conditions can be reached with the first scheme during 6 hours and with the second scheme during 20-30 min.

Organic acids have been obtained in an alkaline medium from a mixture of monosaccharides-carbohydrates to be used in a closed life support system. Methods of analysis of mixtures of synthetic acids have been developed. The composition of mixtures of organic acids has been identified by paper chromatography. During electrodialysis the mixtures release calcium ions. The use of mixtures of synthetic organic acids as carbon source gives rise to an intensive growth of the yeast Candida tropicalis SK-4.