1. [The influence of age of donors on the ability of cells to homologous recombination DNA repair].
- Author
-
Bernadotte A, Mikhel'son VM, Spivak IM, and Ryzhak GA
- Subjects
- Age Factors, DNA Damage, Genomic Instability drug effects, Genomic Instability radiation effects, Humans, Models, Genetic, Mutation, Nucleic Acid Synthesis Inhibitors pharmacology, RNA Polymerase II physiology, Alpha-Amanitin pharmacology, Chromosome Aberrations drug effects, Chromosome Aberrations radiation effects, Recombinational DNA Repair drug effects, Recombinational DNA Repair radiation effects, Tissue Donors, X-Rays adverse effects
- Abstract
Old and young donors cells show different ability to homologous recombination (shown on the first stage--the chromosome transference) in vitro, that we suppose could be the reasons of the genome instability in aging. Homologous recombination, induced by X-radiation, is limited in cells taken from donors older than 70 years. Alpha-amanitin, the RNA-polymerize II repressor, in toxic doze, could induce the chromosome transference in the cells from all studied groups: from old and young donors and donors with repair process defect (with BRCA 1, 2 mutations). Summarized effect of X-radiation and alpha-amanitin does not increase the induction of the chromosome transference.
- Published
- 2013