1. [The influence of methylprednisolone on the ability of CD4 + CD95 + HLA-DR + T-cells to produce proinflammatory medators in cultures of TCR-activated CD3 + CD45RO + T-lymphocytes from patients with rheumatoid arthritis].
- Author
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Todosenko NM, Khaziakhmatova OG, Yurova KA, Malinina IP, and Litvinova LS
- Subjects
- Adult, Antibodies pharmacology, Arthritis, Rheumatoid pathology, CD2 Antigens genetics, CD2 Antigens immunology, CD3 Complex genetics, CD3 Complex immunology, CD4 Antigens genetics, CD4 Antigens immunology, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes pathology, Case-Control Studies, Female, Gene Expression Regulation immunology, HLA-DR Antigens genetics, HLA-DR Antigens immunology, Humans, Interleukin-17 biosynthesis, Interleukin-17 immunology, Interleukin-2 biosynthesis, Interleukin-2 immunology, Interleukins biosynthesis, Interleukins immunology, Leukocyte Common Antigens genetics, Leukocyte Common Antigens immunology, Lymphocyte Activation, Male, Primary Cell Culture, Signal Transduction, Tumor Necrosis Factor-alpha biosynthesis, Tumor Necrosis Factor-alpha immunology, fas Receptor genetics, fas Receptor immunology, Arthritis, Rheumatoid immunology, CD4-Positive T-Lymphocytes drug effects, Gene Expression Regulation drug effects, Glucocorticoids pharmacology, Methylprednisolone pharmacology
- Abstract
The effect of different concentrations of the glucocorticoid (GC) methylprednisolone (MP) on CD4+CD95+HLA-DR+ T-cells and their ability to produce proinflammatory mediators in cultures of TCR-stimulated CD3+CD45RO+ T-lymphocytes in the in vitro system was investigated. T cells were obtained from healthy donors and patients with rheumatoid arthritis (RA).Under conditions of TCR-activation, MP increased the number of CD4+HLA-DR+CD95+ cells in CD3+CD45RO+ cultures obtained from RA patients and did not change their content in the control group. In general, MP decreased production of proinflammatory factors (IFN-, IL-2, IL-17, IL-21 and TNF-) by TCR-activated CD3+CD45RO+ cells from healthy donors and RA, consistent with the overall immunosuppressive mechanism of GC action. The correlation between CD4+CD45RO+HLA-DR+CD95+ T-cell contents and parameters reflecting production of proinflammatory mediators (IL-17, IL-21 and TNF-) in RA patients indicates maintenance of the pro-inflammatory potential of this T-cell population exposed to GC action. We suggest that relative resistance of CD4+CD45RO+CD95+HLA-DR+ T-cells of RA patients to the suppressor effect of GC leads to maintenance and even enhancement in the functional capacities of autoreactive cells in the pathogenesis of RA.
- Published
- 2017
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