1. [High-Content siRNA Screen of the Kinome Identifies Kinases Involved in Git2-Induced Mesenchymal-Epithelial Transition].
- Author
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Cao MG, Xu J, Yang QF, Guo ZP, Zhang KB, Li XB, Wu SQ, and Zhou W
- Subjects
- Animals, Cell Line, Tumor, Epithelial Cells metabolism, Epithelial Cells pathology, Epithelial-Mesenchymal Transition genetics, Female, GTPase-Activating Proteins, Gene Expression Profiling, Gene Expression Regulation, Intercellular Signaling Peptides and Proteins, Mammary Glands, Animal drug effects, Mammary Glands, Animal metabolism, Mammary Glands, Animal pathology, Mice, Protein Kinases classification, Protein Kinases metabolism, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Cell Cycle Proteins pharmacology, Epithelial Cells drug effects, Epithelial-Mesenchymal Transition drug effects, High-Throughput Screening Assays, Phosphoproteins pharmacology, Protein Kinases genetics
- Abstract
Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear. Here, we created a cell based high-content siRNA screen using the breast cancer cell line 4TO7 to search for kinases that were involved in Git2-induced MET. Our results revealed that 58 kinases including transferase, phosphorylation regulators, ATP/nucleotide partners potentially participate in Git2-induced MET. Our preliminary data is expected to facilitate elucidation of the mechanism on how MET is initiated during cancer metastasis.
- Published
- 2017
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