1. [Study of the binding of diuretics by serum proteins according to changes in tryptophan fluorescence].
- Author
-
Lebedev AA and Samokrutova OV
- Subjects
- Alkylmercury Compounds metabolism, Animals, Binding, Competitive, Diuretics blood, Dogs, Drug Combinations metabolism, Ethacrynic Acid metabolism, Fluorescence, Furosemide metabolism, Organomercury Compounds metabolism, Protein Binding, Serum Albumin metabolism, Theophylline metabolism, Tryptophan blood, Blood Proteins metabolism, Diuretics metabolism
- Abstract
The effect of diuretics on tryptophan fluorescence of blood serum proteins divided by electrophoresis was studied. It was shown that ethacrynic acid produces the most significant extinction of tryptophan fluorescence in albumin fraction and novurit in globulin fraction. Furosemide possessing a high affinity for all three obtained fractions of protein does not exhibit a preferential binding to one or another of these fractions. It was also found that furosemide and ethacrynic acid by binding to human serum albumin molecule produce its conformational alterations. Mercury diuretic does not possess such effect.
- Published
- 1989