Search

Your search keyword '"Dymova, M. A."' showing total 5 results
Start Over Author "Dymova, M. A." Language russian
5 results on '"Dymova, M. A."'

3. [Characterization of Recombinant Endonuclease IV from Mycobacterium tuberculosis].

Author

Dymova MA, Endutkin AV, Polunovsky VV, Zakabunin AI, Khrapov EA, Torgasheva NA, Yudkina AV, Mechetin GV, Filipenko ML, and Zharkov DO

Subjects
Amino Acid Sequence, DNA Repair, DNA-(Apurinic or Apyrimidinic Site) Lyase genetics, Deoxyribonuclease IV (Phage T4-Induced), Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins, Mycobacterium tuberculosis genetics, Mycobacterium tuberculosis metabolism
Abstract

Mycobacterium tuberculosis cells contain two apurinic/apyrimidinic (AP) endonucleases, endonuclease IV (MtbEnd) and exonuclease III (MtbXthA), the former playing a dominant role in protecting mycobacterial DNA from oxidative stress. Mycobacterial endonuclease IV substantially differs from its homologs found in Escherichia coli and other proteobacteria in a number of conserved positions important for DNA binding and AP site recognition. The M. tuberculosis end gene was cloned, and recombinant MtbEnd purified and characterized. The protein efficiently hydrolyzed DNA at the natural AP site and its 1'-deoxy analog in the presence of divalent cations, of which Ca^(2+), Mn^(2+), and Co^(2+) supported the highest activity. Exonuclease activity was not detected in MtbEnt preparations. The pH optimum was estimated at 7.0-8.0; the ionic strength optimum, at ~50 mM NaCl. Enzymatic activity of MtbEnd was suppressed in the presence of methoxyamine, a chemotherapeutic agent that modifies AP sites. Based on the results, MtbEnd was assumed to provide a possible target for new anti-tuberculosis drugs.

Published
2021
Full Text
View/download PDF

4. [ANALYSIS OF MUTATION IN CODON 531 rpoB GENE, ASSOCIATED WITH RIFAMPICIN RESISTANCE AMONG SAMPLES OF MYCOBACTERIUM TUBERCULOSIS THAT WAS ISOLATED FROM THE PATIENTS].

Author

Poteĭko PI, Liashenko AA, Lebed' LV, Dymova MA, Kryt'ko VS, Filipenko ML, and Kashuba DA

Subjects
Antitubercular Agents therapeutic use, Codon, DNA-Directed RNA Polymerases, Drug Resistance, Multiple, Bacterial genetics, Humans, Microbial Sensitivity Tests, Mycobacterium drug effects, Mycobacterium isolation & purification, Rifampin therapeutic use, Tuberculosis, Multidrug-Resistant drug therapy, Tuberculosis, Pulmonary drug therapy, Antitubercular Agents pharmacology, Bacterial Proteins genetics, Mutation, Mycobacterium genetics, Rifampin pharmacology, Tuberculosis, Multidrug-Resistant microbiology, Tuberculosis, Pulmonary microbiology
Abstract

In the conditions of high relative density of multidrug resistant tuberculosis in Ukraine, rapid diagnostics of M. tuberculosis susceptibility to antituberculous drugs is the important tool in treatment of patients with lung tuberculosis. 78 patients with lung TB that were treated in TB dispensary in Kharkiv during 2004-2005 years were analyzed. Mutations in 531 codon of rpoB gene of isolates M. tuberculosis were studied. Among 48 (61.5%) resistant isolates of M tuberculosis according to phenotypic method, mutations were found only at 29 (60.4%) isolates. Among 30 (38.5%) phenotypic sensitive M. tuberculosis isolates only 1 (3.3%) isolate had mutation in rpoB gene M. tuberculosis.

Published
2014

5. [The first results of a combined all-Russian study on clinical genetics of multiple sclerosis].

Author

Sokolova EA, Malkova NA, Korobko DS, Rozhdestvenskiĭ AS, Kakulia AV, Khanokh EV, Delov RA, Platonov FA, Popova TE, Aref'eva EG, Zagorskaia NN, Alifirova VM, Titova MA, Smagina IV, El'chaninova SA, Popovtseva AV, Lichenko IuN, Dymova MA, Vaĭner AS, P'iankova OV, Oskorbin IP, Kechin AA, Puzyrev VP, Kulakova OG, Tsareva EIu, Favorova OO, Shur SG, Lashch NIu, Popova NF, Gusev EI, Boĭko AN, Aul'chenko IuS, and Filipenko ML

Subjects
Adult, Alleles, CD40 Antigens blood, Female, Gene Frequency, Genotype, Humans, Incidence, Male, Multiple Sclerosis blood, Multiple Sclerosis epidemiology, Polymerase Chain Reaction, Retrospective Studies, Risk Factors, Russia epidemiology, CD40 Antigens genetics, DNA genetics, Genetic Predisposition to Disease, Multiple Sclerosis genetics, Polymorphism, Genetic, Risk Assessment methods
Abstract

Multiple sclerosis is a classic multifactorial disease in which etiology interaction of external factors and structural features of a large number of genes plays an important role. Identifying risk factors for multiple sclerosis and creating an integrated model of pathogenesis are urgent tasks of neurology. Revealing true risk factors is possible only in studies with sufficient statistical power, so with a large amount of samples. We conducted the association study of CD40 gene's polymorphisms and multiple sclerosis among residents of the Russian Federation. The results demonstrated the need to combine data from different researchers in clinical studies to increase the power of the study.

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results