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The etiological role of conditionally pathogenic bacteria in acute intestinal infections in the Sumy region has been studied. It is established, that the strains of Klebsiella pneumoniae, Staphylococcus aureus and Enterobacter cloacae isolated from patients with acute intestinal infection were characterized by phenotypic heterogeneity. In 85.0 +/- 5.6% of the studied strains of K. pneumoniae, 30.0 +/- 7.2% of E. cloacae, 20.0 +/- 5.6% S. Aureus the authors have identified pathogenicity factors, which ensure their ability to adhesion and persistence (antiinterferon and anticomplement activity).

To determine the prevalence,and etiological structure of acute intestinal infections, to investigate the dominant agents' persistence factors. According with materials of statistical reports we did the retrospective epidemiological analysis of acute intestinal infections incidence in Sumy region from 2006 till 2011. Biological properties of 40 strains of Klebsiella pneumonia, 40 strains of Enterobacter cloacae and 50 strains of Staphylococcus aureus were investigated. Moderate trend of acute intestinal infections incidence increase was indicated. Bacteria of genera Klebsiella, Enterobacter, Staphylococcus were predominated in etiological structure. Incidence of acute diarrheal infections caused by Klebsiella and Enterobacter was reached the maximum in the spring-summer period. The incidence of staphylococcal etiology was discrete. The strains of Klebsiella pneumonia, Staphylococcus aureus and Enterobacter cloacae were remarkable for different frequency and intensity of persistence factors. Аnti-interferon activity was detected in 100% of clinical isolates of microorganisms, anti-lysozym activity was detected in 87.3 ± 2.9% of clinical isolates of microorganisms, anti-complementary activity was detected in 72.3 ± 3.9% of clinical isolates of microorganisms. Biological properties of opportunistic pathogens that cause acute intestinal infections can be used as epidemiological factors for differentiation of microorganisms pathogenicity.

Susceptibility of nosocomial potential AmpC-beta-lactamases producers strains (n=128), isolated from patients admitted to 30 medical centers of 15 various regions of Russia has been investigated. The susceptibility testing was performed by the broth microdilution method. The most active antibacterial agents acting to the investigated strains remained carbapenems (imipenem and meropenem). PCR-based detection of beta-lactamase genes (TEM, SHV, CTX) was investigated in 51 E. cloacae strains. Alone or in various combinations TEM type beta-lactamases have been found in 31 (60.8%) isolates, SHV in 22 (43.1%), and CTX--in 22 (43.1%). There were negative results of TEM, SHV, CTX beta-lactamases genotyping in 13 (25.5%) E. cloacae suspect strains.

A total of 120 strains of E. cloacae were isolated from colonized newborns in a maternity hospital (96 strains) and from inpatients of other hospitals (23 strains). Biovars of these strains and of 1 reference strain were determined by two methods of biochemical typing and their sensitivity to 14 antibiotics assessed. The overwhelming majority of isolates from newborns were referred to type 2 according to typing after Old (original) or to biovar 62 if typed using the modification of this method. The phenotypical profile of determinants of resistance to 10 antibacterial drugs were the same, including those to aminoglycosides (except amikacin) and third-generation cephalosporins. The strains isolated from non-obstetrical inpatients belonged to biovars with phenotypical profiles of resistance to only 3 antibiotics and sensitive to aminoglycosides and third-generation cephalosporins.