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Your search keyword '"Gene Products, tat metabolism"' showing total 2 results
Start Over Descriptor "Gene Products, tat metabolism" Language russian
2 results on '"Gene Products, tat metabolism"'

1. [Cytotoxicity of chimera peptides incorporating sequences of cyclin kinases inhibitors].

Author

Kharchenko VP, Kulinich VG, Lunin VG, Filiasova EI, Shishkin AM, Sergeenko OV, Riazanova EM, Voronina OL, and Bozhenko VK

Subjects
Amino Acid Sequence, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p16 biosynthesis, Cyclin-Dependent Kinase Inhibitor p16 genetics, Cyclin-Dependent Kinase Inhibitor p21 biosynthesis, Cyclin-Dependent Kinase Inhibitor p21 genetics, Gene Expression Regulation, Neoplastic, Genes, p16, Genes, p53, Humans, Jurkat Cells, Molecular Sequence Data, Peptides pharmacology, Antennapedia Homeodomain Protein metabolism, Apoptosis drug effects, Chimera, Cyclin-Dependent Kinase Inhibitor p16 pharmacology, Cyclin-Dependent Kinase Inhibitor p21 pharmacology, Gene Products, tat metabolism
Abstract

The study is concerned with proapoptotic properties of chimera peptides which incorporate sequences of inhibitors of cyclin kinases p161NK4a and p21CIP/WAF1 as well as internalized sequences (Antp and tat). Sequences of the p16 type appeared to be more cytotoxic than the p21 one. Cytotoxic effect proved dependent on orientation with respect to the C or N terminal point of a polypeptide chain rather than on chimera sequence extent. Although p16 endogenous synthesis did not influence chimera peptide levels, apoptosis did not take place in certain cellular lines. Due to the rather unsophisticated nature of such synthesis, it might be used in designing individually-tailored chemotherapeutic drugs.

Published
2007

2. [Influence of regulatory genes of type 1 human immunodeficiency virus on proliferation and differentiation of murine embryonic stem cells].

Author

Manuilova ES, Arsen'eva EL, Khaĭdarova NV, Shugurova IM, Gornostaeva SN, Inozemtseva LS, Katrukha AI, Grivennikov IA, and Tarantul VZ

Subjects
Animals, Cell Division genetics, Cells, Cultured, Cytomegalovirus genetics, Embryo, Mammalian cytology, Gene Products, nef metabolism, Gene Products, tat metabolism, Genes, Regulator, Genes, Viral, Green Fluorescent Proteins, Luminescent Proteins genetics, Luminescent Proteins metabolism, Mice, Mice, Inbred Strains, Myocytes, Cardiac cytology, Promoter Regions, Genetic genetics, Recombinant Proteins genetics, Recombinant Proteins metabolism, Stem Cells physiology, Transfection, nef Gene Products, Human Immunodeficiency Virus, tat Gene Products, Human Immunodeficiency Virus, Cell Differentiation genetics, Gene Products, nef genetics, Gene Products, tat genetics, HIV-1 genetics, Stem Cells cytology
Abstract

Spontaneous formation of embryoid bodies and subsequent differentiation of some cells into cardiomyocytes were demonstrated on murine embryonic stem cells of R1 line. The lines of embryonic stem cells were obtained that had been transfected with genetic constructs carrying expressing regulatory genes of the human immunodeficiency virus tat and nef and "green protein" gene (GFP). The transfection of embryonic stem cells with the gene tat stimulated their proliferative activity, while this activity decreased in the cells transfected with the gene nef. The time necessary for the formation of embryoid bodies by all lines of transfected cells was similar to that in the control cells. In the cultures of cells transfected with nef and tat, the number of embryoid bodies and the percentage of embryoid bodies with contracting cardiomyocytes were higher and lower than in the control, respectively. Thus, an inverse correlation was observed between the effects of regulatory genes of the human immunodeficiency virus on proliferation and differentiation embryonic stem cells.

Published
2003

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