1. [Characterization and expression of DjPreb gene in the planarian Dugesia japonica].
- Author
-
Yuan ZQ, Zhao BS, Zhang JY, and Zhang SC
- Subjects
- Animals, DNA-Binding Proteins classification, Gene Expression, Gene Expression Profiling, Guanine Nucleotide Exchange Factors classification, Helminth Proteins classification, Phylogeny, Planarians growth & development, Regeneration genetics, Sequence Analysis, DNA, Transcription Factors classification, DNA-Binding Proteins genetics, Gene Expression Regulation, Developmental, Guanine Nucleotide Exchange Factors genetics, Helminth Proteins genetics, Planarians genetics, Transcription Factors genetics
- Abstract
In this study we report the expression and identification of a PREB-related gene from the planarian Dugesia japonica, DjPreb. The planarian DjPreb cDNA is comprised of 1101 bp and contains a 972 bp open reading frame corresponding to a deduced protein of 323 amino acids with a 69 bp 5'-UTR and a 60 bp 3'-UTR. Phylogenetic analysis shows that DjPreb belongs to PREB/PREB-like members. We examined its spatial and temporal expression and distribution in both intact and regenerating planarians by Relative quantitative real-time PCR and Whole-mount in situ hybridization. The analysis indicates that DjPreb shows a gradient of expression with peak levels present in the anterior and posterior regions and progressively lower levels in central regions in intact and regenerating planarians. During regeneration the expression of DjPreb is upregulated. Strong expression of DjPreb is observed in the anterior and posterior blastemas. These results suggest that DjPreb may participate in head and tail formation.
- Published
- 2010