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The aim of the work was to determine the possibility of using the powder from the leaves of Hippophae rhamnoides L. for enriching flour confectionery and to evaluate the antimutagenic and antioxidant activity of the product. The experiment was carried out on 24 white Wistar rats with initial body weight (b.w.) 180-200 g. The animals of the experimental group (n=8) received confection containing sea buckthorn powder at a rate of 20 mg per 100 g b.w. for 14 days on the background of a standard vivarium diet. The animals of the control and intact groups received confection containing no bioactive supplement at the same dose. Antimutagenic and antioxidant effects were estimated in a day after a single injection of cyclophosphamide at a dose of 20 mg/kg b.w. The number of chromosomal aberrations in bone marrow cells of white rats was counted and the activity of catalase, superoxide dismutase (SOD), the level of reduced glutathione and the concentration of TBA-active products in blood were evaluated. The intake of the confectionery containing the powdered H. rhamnoides leaves resulted in the 45% decrease of the number of damaged cells, 50% decrease of the proportion of cells with multiple chromosome breaks and 52% decrease of the number of achromatic gaps as compared to animals of the control group (n=8). The cake intake increased the activity of catalase (by 52%) and SOD (by 33%) and glutathione content (by 26%) in blood., (Copyright© GEOTAR-Media Publishing Group.)

Nettle extract produced from leaves crushed to 40-70 nm fragments protects the stomach mucous membrane, and does it better than the extract derived from same leaves crushed to 1 mm fragments, on the models of peptic ulcers caused by acetylsalicylic acid, histamine, prednisolone, and immobilized stress. The antiulcer activity of the nettle extract from 40-70 nm fragments is comparable with the effect of buckthorn oil. Nettle extracts also hinder the excess acid secretion and diminish the acidity of stomach juice in experimental peptic ulcer caused by pylorus ligation.

Results of phytochemical analysis of the berries from six Russian Hippophae rhamnnoides L. varieties (Augustinka, Botanitseskaja, Ljubitelskaja, Vorobjvskaja, Pertsik, Trofimovskaja), cultivated in Finland, are presented. The contents of ascorbic acid (vitamin C), tocopherols (vitamin E), total flavonoid (vitamin P), total carotenoid (vitamin A) and total organic acids were analyzed in unfreezed berries. The same parameters were defined in food products (jelly, sweet sauce and compote) containing fruits of three Hippophae rhamnoides L. varieties (Botanitseskaja, Ljubitelskaja and Trofimovskaja). According to the results, Russian Hippophae rhamnoides L. varieties can be cultivated in South-Finland and different kinds of food products with high quality and concentration of biologically active compounds may be prepared from these berries.

Gastrobiol--a new preparation comprising a mixture of lyophilized sea-buckthorn oil, vitamin U, and magnesium oxide, produces a pronounced antiulcerous action on the mucous membrane of the stomach and duodenum. The regenerative effect of gastrobiol is more pronounced than that of pure sea-buckthorn oil and methyluracil.

We studied the influence of the synchronized signals of action (SSA) that were formed in human organism by natural biological antioxidants from sea-buckthorn (Prielbrusie) on indices of the blood and cardiovascular system. We have shown that the use of the natural biological antioxidants during 10 days normalized SaO2 fluctuation, pulse waves and blood distribution in tissues. SSA that they formed influence in the same way on recipients that were at the large distance from donor, influences were registered through the short time--6 min. We suggested a hypothesis about the distant control of physiological functions and organism adaptation processes through SSA, that were formed at the cell energetic and informational level by natural factors (biological antioxidants, adaptation to high mountains). We suppose that successes in this direction of science will have not fundamental importance only but social and practical as well.