1. [Beta-1 and beta-4 integrins, and laminin 67 kDa receptors in interaction between A431 cells and laminin isoforms ].
- Author
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Cherepanova OA, Kalmykova NV, Blinova MI, and Pinaev GP
- Subjects
- Basement Membrane physiology, Cell Adhesion physiology, Cell Line, Tumor, Humans, Integrin beta1 physiology, Integrin beta4 physiology, Protein Isoforms metabolism, Receptors, Laminin physiology, Integrin beta1 metabolism, Integrin beta4 metabolism, Laminin metabolism, Receptors, Laminin metabolism
- Abstract
Laminins, as basal membrane glycoproteins, are able to stimulate cell adhesion and migration, and to influence gene expression. The laminin molecule has a set of bioactive sites that interact with different integrin and nonintegrin receptors, and, as a result, the reaction of the same cell type to different laminin isoforms may be different. The aim of this study was to determine the contributions of both integrins with beta 1 and beta 4 chains and 67 kDa laminin receptor in the interaction of A431 cells with two laminin isoforms: laminin-1 and laminin-2/4. The obtained data show that integrin alpha 6 beta 4 is more specific for interaction with laminin-2/4 than with laminin-1 and takes part in the stage of attachment of A431 cells to laminin. 67 kDa receptor promotes cell spreading on laminin-2/4 and inhibits cell spreading on laminin-1. An assumption was made about the complex action of receptors for interaction of A431 cells with laminins ("integrin alpha 6 beta 4--67 kDa receptors" complex).
- Published
- 2003