1. [Synthesis and properties of methylene carboxamide mimetics of nucleic acids based on morpholine nucleosides].
- Author
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Abramova TV, Kasakin MF, Tarasenko IuV, Lomzov AA, Koval' VV, Pyshnyĭ DV, and Sil'nikov VN
- Subjects
- Adenine chemistry, Nucleic Acid Conformation, Uracil chemistry, Morpholinos chemical synthesis, Morpholinos chemistry, Morpholinos isolation & purification, Nucleic Acids chemical synthesis, Nucleic Acids chemistry, Oligodeoxyribonucleotides chemical synthesis, Oligodeoxyribonucleotides chemistry, Oligodeoxyribonucleotides isolation & purification
- Abstract
Uracyl and adenine containing oligocarboxamide mimetics of nucleic acids based on morpholine nucleosides (MorGly) are synthesized using peptide chemistry methods. Conditions for an analysis of homogeneity of protonated at physiological pH oligomers using a capillary electrophoresis are proposed. Studies of thermostability of complementary complexes formed by MorGly oligomers revealed that melting temperature dramatically depends on heterocyclic base composition (uracyl or adenine). Cooperative interactions realized at junctions in tandem complexes give more contribution to the thermostability in the case of complexes formed by modified oligomers than native oligodeoxyriboadenilates. Adenine containing MorGly oligomers form more stable complexes with poly(U) than native oligodeoxyriboadenilate of the same length. Complexes formed by modified oligomers with polyribonucleotides are more stable in compare with polydeoxyribonucleotide.
- Published
- 2012
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