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Fruit aroma is an important consumer trait of strawberry varieties. It is accumulated in the fruit during ripening and is due to a combination of large quantities of volatile aroma-forming organic compounds. One of the most important aroma-forming compounds is anthranilic acid methyl ester (methyl anthranilate). However, many modern strawberry varieties are characterized by low methyl anthranilate content in fruits. The purpose of the study was marker-assisted screening of strawberry varieties from the genetic collection of FSSI “I. V. Michurin Federal Scientific Center” (hereinafter “I. V. Michurin FSC”) by the FanAAMT gene involved in the control of methyl anthranilate determination (encodes anthranilic acid methyltransferase) to identify forms promising in breeding for fruit aroma. The studies were carried out in 2022–2023. Strawberry varieties (Fragaria × ananassa Duch.) created at “I. V. Michurin FSC”, as well as those introduced from various ecological and geographical regions of origin, were used as biological objects. Target allele of the FanAAMT gene in strawberry genoplasm was identified by FanAAMT primers. Wild species of Fragaria vesca L. served as a positive control. As a result of the research, the 1500 bp target amplicon, corresponding to the functional allele of the FanAAMT gene, was identified in 38.1 % of studied forms. Among the Russian strawberry varieties, the FanAAMT gene was present in 44.4 % of genotypes, among varieties of foreign breeding – in 33.3 % of forms. No statistically significant differences between the samples of Russian and foreign varieties were found (tfact = 0.3 ≤ tst = 4.3). The sources of the functional allele of the FanAAMT gene for breeding are varieties ‘Elizaveta 2’, ‘Rubinovyy kaskad’, ‘Slavutich’, ‘Amy’, ‘Joly’, ‘Limalexia’ and ‘Rumba’. Strawberry variety ‘Lastochka’ (922-67 × ‘Privlekatelnaya’), created at “I.V. Michurin FSC”, is a complex source of fruit aroma genes, namely FanAAMT (methyl anthranilate biosynthesis) and FaOMT (mesifurane biosynthesis). In the rest of the analyzed strawberry genotypes, target amplicon of the marker FanAAMT was not detected indicating the absence of FanAAMT gene.