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The authors followed up (in outpatient conditions) a total of fifty 1-to-9-mounth-old infants with the diagnosis of atopic dermatitis, disseminated form, of subacute course. The infants were followed up for 3-12 months, with 83% of the 113 examined patients 1-3 weeks after the beginning of treatment having shown clinical improvement of cutaneous inflammatory processes. Of the 172 infants subjected to immunological examination, in 100--the level of total IgE in blood serum was significantly elevated. These children also had elevated level of the specific IgE antibodies to cow's milk proteins. The outcomes of the carried out dietotherapy using mixture "Nanny" showed that this therapeutic product resulted in remission in the majority of the followed up infants suffering from allergy to cow's milk proteins.

Some samples of cow milk proteins and enzymatic hydrolysates prepared on their basis were investigated in experiments on male guinea pigs to elucidate their sensitizing properties and influence on sensitivity to histamine LD50. The data obtained are discussed with respect to a possible modulation of allergic reactions by the products of incomplete enzymatic proteolysis.

Altogether 54 children with food allergy characterized clinically by concomitant lesion of the skin and alimentary tract were examined. The results obtained attest to the involvement into the pathological process of the small intestine, to the combination of cow's milk protein and lactose intolerance as well as to the advisability of correcting intestinal dysbiocenosis in order to reduce the disease progress.

Ultrastructural studies and counts of interepithelial lymphocytes and the density of cellular infiltration of the lamina propria were carried out on 10 biopsies of the small intestine from infants of 4 months to 2 years with the syndrome of disturbed intestinal absorption. The greatest disorders were found to occur in celiac disease and intolerance to cow milk protein and to be manifested in subtotal atrophy of villi and destructive lesions of enterocytes. The authors believe that the marked infiltration of the epithelial layer with lymphocytes and the lamina propria of the mucous membrane with plasma cells represent manifestations of the local immunological response.

Adult guinea pigs received intragastrically solutions of milk whey protein concentrate (CWPM), its enzymatic hydrolysate (HWPM) and peptide fraction (PF) obtained by means of HWPM ultrafiltration. Milk protein antigen content of CWPM, HWPM and PF was preliminary characterized with two-dimensional and rocket immunoelectrophoresis. Undigested beta-lactoglobulin (beta LG) absorbed from the gut, was measured three hours after the administration by means of competitive radioimmunoassay. Administration of proteins was repeated twice with daily intervals. Twenty one days later the levels of circulating anti-beta LG antibodies were measured by Farr's method and then the reaction of active anaphylactic shock was triggered by intravenous infusion of whole skimmed milk. It was established that while antigens including beta LG content decreased in the rank of CWPM-HWPM-PF the absorption of beta LG into the blood serum and the violence of acute anaphylaxis decreased also. The largest level of specific antibodies to beta LG was detected in animals that received the hydrolized protein the fact being connected with the presence of aggregated beta LG in this preparation. The employment of ultrafiltration of enzymatic hydrolyzate leads to elimination of the majority of noninactivated antigens from the filtrate, consequence of this being the low level of animal sensitization by this preparation. It has been concluded that ultrafiltration technology has good advances in manufacturing hypoallergenic milk formulae.