1. [THE STUDY OF THE ORIGIN OF HUMAN GLIOMA BASED ON CELL LINES AND TUMOR SPECIMENS].
- Author
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Timin GV, Lakhina YS, Gulyaev DA, and Tolkunova EN
- Subjects
- Astrocytoma metabolism, Astrocytoma pathology, Biomarkers metabolism, Brain Neoplasms metabolism, Brain Neoplasms pathology, Cell Line, Tumor, Gene Expression, Glioblastoma metabolism, Glioblastoma pathology, Humans, Neoplasm Proteins metabolism, Neoplastic Stem Cells metabolism, Neoplastic Stem Cells pathology, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Neurocytoma metabolism, Neurocytoma pathology, Octamer Transcription Factor-3 genetics, Octamer Transcription Factor-3 metabolism, Oligodendroglioma metabolism, Oligodendroglioma pathology, Primary Cell Culture, Proto-Oncogene Proteins c-kit genetics, Proto-Oncogene Proteins c-kit metabolism, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells cytology, Stem Cells metabolism, Astrocytoma genetics, Brain Neoplasms genetics, Glioblastoma genetics, Neoplasm Proteins genetics, Neurocytoma genetics, Oligodendroglioma genetics
- Abstract
The present study is dedicated to the search of human glioma "cells of origin". Specimens of the tumor tissue have been analyzed via the RT-PCR method to potentially reveal the expression of molecular markers characteristic of various cells of nerve tissue (NeuN, MOG, MBP, NG2, Olig2, Vimentin, GFAP, AldhlL1), as well as markers of stem (Oct4, c-Kit) and cancerous stem (CD133) cells. We have shown that the expression profiles of these markers for different types of gliomas intersect, which does not allow to determine the type of "cells of origin". So, in order to study the origin of glioma using cell lines derived from primary cultures, we need a more sophisticated culture conditions, rather than the commonly used serum-based media.
- Published
- 2015