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Hydrocephalus is a central nervous system disorder which most commonly affects infants and toddlers. It starts as an abnormal build-up of cerebrospinal fluid in the ventricular system of the brain. Hence, early diagnosis becomes vital, which may be performed by Computed Tomography (CT), one of the most effective diagnostic methods for diagnosing Hydrocephalus (CT), where the enlarged ventricular system becomes apparent. However, most disease progression assessments rely on the radiologist's evaluation and physical measures, which are subjective, time-consuming, and inaccurate. This paper develops an automatic prediction utilizing the H-detect framework for enhanced accurate hydrocephalus prediction. This paper uses a pre-processing step to normalize the input image and remove unwanted noises, which can help extract valuable features easily. The feature extraction is done by segmenting the image based on edge detection using triangular fuzzy rules. Thereby, the exact information on the nature of CSF inside the brain is highlighted. These segmented images are saved and again given to the CatBoost algorithm. The Categorical feature processing allows for quicker training. When necessary, the overfitting detector will stop model training and thus efficiently predicts Hydrocephalus. The outcomes demonstrate that the new H-detect strategy outperforms the traditional approaches.

Bloodstream infections (BSI) are associated with high mortality rates, especially in immunocompromised patients. Identifying pathogens early and selecting appropriate antimicrobials to treat BSI is integral in reducing the mortality rate. There is a need to reduce the turnaround time (TAT) of pathogen identification as well as to accelerate the antimicrobial susceptibility testing (AST) of blood cultures, which can be achieved by following relevant identification methods and performing the direct AST (DAST) by the disk diffusion method. In this study, blood samples were collected from patients with suspected bacteremia/septicemia, and aseptic precautions were taken to prevent contamination. Samples containing gram-negative bacilli (GNB) were then analyzed by DAST and conventional AST (CAST). We tested 118 GNB-positive isolates in total to compare the results of DAST and CAST. DAST and CAST showed good categorical agreement (CA) for various groups of microorganisms: 98.9% and 99.6% for Enterobacterales and Pseudomonas spp., respectively. Early detection of pathogens in blood along with the determination of their antibiotic susceptibility patterns is a need of the hour. By performing DAST on positive blood culture broth, clinical teams can obtain the information necessary for switching from empirical therapy to definitive treatment one day faster. This rapid identification of the pathogen, along with corresponding AST results, will help clinicians to accelerate targeted antimicrobial therapy for critical patients and, thus, reduce mortality and morbidity rates in patients with bloodstream infections.

On entering formal education, infants face the demand of participating in collective educational rou¬tines and learning experiences. However, in this age period, the sense of collectiveness is still in an embry¬onic form. This study explored how infants enter into and experience the need for collectiveness and how teachers create the conditions for the development of a sense of collectiveness during infancy. Our educa¬tional experiment drew on a Conceptual PlayWorld, as a collective model of practice for the development of play and imagination. Thirteen infants (0,5—2 years old) participated in the study. Visual methods were used for digital data collection and analysis. It was found that, being in the imaginary situation as play part¬ners, teachers introduced to the infants’ environment the demand to align with the collective, consistently facilitated and sustained infants’ motive orientation to the collective. The use of props, the embodiment of the experience and the shift from physical objects and concrete spaces to a shared intellectual and abstract space appeared to be critical. The findings inform everyday practice and policy opening up a new area of understanding about the concept of collective imagining, as an important concept for the development of a collective orientation for infants.

Autoimmune categorized as a dangerous non-communicable disease that tends to be at bigger risk for women and children. Lack of understanding on general public and medical experts has an impact to the high mortality rate from autoimmune diseases.Having a response to the fact, Marisza Cardoba Foundation as a non-governmental organization concerned with health inaugurated the Senyum Indonesiaku National Program along with the Ministry of Women's Empowerment and Child Protection to support public awareness of autoimmune. The purpose of this research is to understand and analyze the implementation of one of the program strategies. This study uses Harold Laswell's communication theory and public relations campaign by Ronald D. Smith.The research method used is a descriptive-qualitative approach. The research finding found that Idolanesia Awards Indonesia 2017as one of the strategies for Senyum Indonesiaku National Program, include as good effort to support public awareness regarding the autoimmune. However, weaknesses were still found in the planning strategies of the program.

Digital technologies have created possibilities in research unavailable when Vygotsky first introduced his cultural-historical approach for studying children’s development. More needs to be known about the relations between methodology and method when using digital tools in the early developmental period (1-5 years). In this paper we introduce the concept of a living laboratory to capture the research dynamics of this cultural age period in family homes and preschool settings under conditions of an educational experiment. We discuss Vygotsky’s theoretical concepts as foundational for theorising the use of digital tools for researching in a living laboratory. Central for a living laboratory are: (1) capturing development in motion, (2) including the past in the present research context, (3) designing studies in ways that go beyond fossilised complete forms of development, and (4) creating study conditions for condensed and amplified forms of development. To bring these conditions into the research contexts where a condensed form of development emerges opens up a dynamic yet dialectical way of studying early development. We showcase digital tools, such as VR and digital data collection, as part of (1) undertaking an educational experiment of a Conceptual PlayWorld, and (2) a cultural-historical conception of longitudinal research for studying the conceptual development of infants, toddlers and preschoolers within a living laboratory.

BACKGROUND: Diabetes Mellitus (DM) is a common metabolic disease worldwide. Electrolyte played significant roles in the normal functioning of the body, and deregulation is indicative of different types of disease and electrolyte disturbances are often reported in type 2 DM (T2DM). AIM: The aim of the study was to estimate the levels of serum electrolytes in outpatients with T2DM and correlate serum electrolytes with random blood sugar (RBS). MATERIALS AND METHODS: Patients with T2DM visiting the outpatient Departments of Medicine, between April 2016 and March 2017 were included. Of 148 diagnosed T2DM cases, 74 were had RBS level >300mg/dL (group-1) and 74 had RBS level ≤300mg/dL (group-2). Serum sodium (Na+), potassium (K+), chloride (Cl-) levels were measured by using the Roche 9180 electrolyte analyzer. RESULTS: In this study, there was a significant decrease in serum Na+ levels in group 1 (131.83±4.36 mmol/L) compared to group 2 (134.15±4.90 mmol/L).The serum levels of K+ was found to be increased in group 1 (4.51±0.61 mmol/L) in comparison with group 2 (4.26±0.52 mmol/L). In group-1, an inverse relationship was present between serum Na+ (r=-0.342) and Cl- (r=-0.538) with RBS which was statistically significant. In group-2, a significant correlation was present between serum K+ and RBS (r=0.356, p

Vitamins are the functional components of various enzyme-regulated biochemical reactions occurring to create energy. Vitamin contents of plants are known to show altered metabolism under the influence of salinity. Not much of work has been done on the influence of salinity on the vitamin content in higher plants. Present study was carried out to study the influence of NaCl salinity on vitamin content in the leaves of Spinacia oleracea. Spinacia oleracea plants were grown in earthen pots and were subjected to different concentrations of saline water (NaCl) treatment. Control plants were irrigated with tap water. Treatments started after the seedling emergence and continued till the plants were 45 day old. Mature leaves of these plants were harvested and used for studies. Thiamine and riboflavin content were found to increase with increase in NaCl concentration, however, β-carotene was found to decrease with increasing level of NaCl in the growth medium.

The crop yield is reduced under saline conditions and this hampers agricultural productivity. The incorporation of plant growth regulators (PGRs) during presoaking treatments in many crops has improved seed performance under saline conditions. In order to study the ameliorative effect of plant growth regulators, experiments were conducted to study the variation in organic constituents in the leaves of Trigonella foenum-graecum L. var.PEB, where the seeds were primed with different plant growth regulators and grown under NaCl salinity. After a pre-soaking treatment of six hours in 20 mg L-1 solutions of gibberllic acid (GA3), 6-furfuryladenine (Kinetin) and benzyl adenine (BA), the seeds were allowed to germinate and grow for forty-five days under saline conditions. On the analysis of mature leaves, it was observed that chlorophyll a and b, total chlorophyll and protein showed an increase in PGR-treated plants compared to the untreated set. The accumulation of the stress metabolite such as proline and sugars, which increase under saline conditions, showed a significant decrease in the plants pretreated with PGRs.

The influence of the laser-pulse radiation defocusing on the formation of the desired component and charge composition of the surface plasma is studied using the laser spark spectrometry (LSS-1 spectrometer). The possibility for increasing the portion of AlO and AlN radicals in the plasma when the aluminum target is subjected to the effect of the successive defocused double laser pulses is shown.

Vitamin contents of plants are also known to show altered metabolism under the influence of salinity. Not much of work has been done on the influence of salinity on the vitamin content in higher plants. The influence of NaCl salinity on the vitamin content in the leaves of Atriplex hortensis was investigated in the present study. Atriplex hortensis plants were grown in earthen pots and were subjected to different levels of saline water (NaCl) treatment. Control plants were irrigated with tap water. Treatments started after the seedling emergence and continued till the plants were 30 day old. Mature leaves of these plants were harvested and used for studies. β -carotene, a precursor of vitamin A and ascorbic acid content were found to increase gradually with increase in the concentrations of NaCl. No significant changes were observed in thiamine and riboflavin content at lower levels of salinity, however significant decrease was observed in thiamine and riboflavin content at higher level of salinity.

Plants respond to the environmental cues in various ways, recent knowledge of RNA interference in conferring stress tolerance had become a new hope of developing tolerant varieties. Here we attempt to unfold the molecular mechanism of stress tolerance through miRNA profiling and expression analysis in Finger millet (Eleusine coracona) under salt and drought stress conditions. The expression analysis of 12 stress specific conserved miRNAs was studied using semi-quantitative real time PCR and Northern blot assay. Our studies revealed that, although most of the miRNAs responded to the stresses, the expression of particular miRNA differed with the nature of stress and the tissue. The expression analysis was correlated with the existing data of their target genes. Abiotic stress up-regulated miRNAs are expected to target negative regulators of stress responses or positive regulators of processes that are inhibited by stresses. On the other hand, stress down-regulated miRNAs may repress the expression of positive regulators and/or stress up-regulated genes. Thus the current study of miRNAs and their targets under abiotic stress conditions displays miRNAs may be good candidates to attribute the stress tolerance in plants by transgenic technology.

Exposure to environmental stress due to salinity has been reported to result in adverse effects on the growth of plants. Studies have shown that the use of plant growth regulators (PGRs) has an ameliorative effect on plants grown under saline conditions. In the present investigation, effect of gibberellic acid (GA3), 6-furfuryladenine (Kinetin) and benzyl adenine (BA) on Spinacia oleracea L. var. All Green, cultivated under saline conditions has been studied. After a pre-soaking treatment of six hours in 20 mg L-1 solutions of GA3, Kinetin and BA, the seeds were allowed to germinate and grow for forty-five days under saline conditions. On the analysis of mature leaves, it was observed that both chlorophyll a and b, and total chlorophyll showed an increase in PGR-treated plants compared to the untreated set. With the pretreatment, the reducing and non-reducing sugar content, as well as protein content of the leaves showed an increase in accumulation compared to the untreated plants. The accumulation of the stress metabolite proline, which increases under saline conditions, showed a significant decrease in the plants pretreated with PGRs.

Myrosinase is an enzyme of cruciferous vegetables, hydrolyse glucosinolates. The breakdown products are involved in plant defence against insect and also have anti-fungal property. Myrosinase has been purified to apparent homogeneity from 5 days old germinated cauliflower seedlings having a specific activity of 12.71 units/mg proteins with 54.6 % recovery, using ammonium sulfate fractionation followed by gel filtration chromatography on Sephadex G-100. Effect of some metal ions and carbohydrates on the activity of partially purified cauliflower myrosinase was studied. Sr+2 at 4 mM concentration exhibited marked activating effect on the activity up to 2.7 fold while Fe+2 significantly inhibited. However, Sn+2 and Ba+2 increased the activity to a certain extent and then suppressed. On the other hand, some metal ions [Fe+2, Fe+3, Cu+2 and Zn+2] strongly inhibited the activity even at lower concentrations. Several carbohydrates viz., glucose, fructose, sucrose, maltose and sorbitol even at comparatively higher concentrations had little detectable inhibitory effects. Activation kinetics of myrosinase in presence of Sn+2 and Sr+2 were studied between 0- 20min. The rate of reaction was almost constant till 15 min and then slight deactivation was recorded at various concentrations used.