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1. [Nucleotide sequence of internal transcribed spacers and 5.8S rDNA for the ribosomal operon from alfalfa Medicago sativa and cotton Gossypium hirsutum L].

Author

Mukhamedov RS, Martsinkovskaia AI, Shu Kh, and Abdukarimov AA

Subjects
Base Sequence, Cloning, Molecular, DNA Primers, Molecular Sequence Data, Sequence Homology, Nucleic Acid, DNA, Ribosomal genetics, Gossypium genetics, Medicago sativa genetics, Operon, RNA, Ribosomal, 5S genetics
Abstract

The 708- and 769-bp fragments from alfalfa and cotton containing the 3'-end of the 18S gene, the internal transcribed spacer. 1 (ITS1) the 5.8S gene, ITS2, and the 5'-end of the 28S gene were obtained using primers to the 18S and 28S ends of rDNA from tomato by a polymerase chain reaction. These sequences were cloned into pTZ19R. The 5.8S rDNA, ITS1 and ITS2 nucleotide sequences of alfalfa and cotton were determined. Comparative analysis of nucleotide sequences of alfalfa and cotton showed large hypervariability of spacer regions and conservatism of 5.8S rDNA sequence.

Published
1994

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