1. [Reparative regeneration of connective tissue structures of mammals under antioxidant therapy conditions].
- Author
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Belova SV, Norkin IA, and Puchin'ian DM
- Subjects
- Animals, Arthritis, Experimental metabolism, Arthritis, Experimental pathology, Blood Sedimentation drug effects, Chinchilla, Connective Tissue drug effects, Connective Tissue metabolism, Connective Tissue pathology, Drug Therapy, Combination, Glycosaminoglycans metabolism, Hexoses metabolism, Injections, Intra-Articular, Male, Malondialdehyde metabolism, Stifle drug effects, Stifle metabolism, Stifle pathology, Synovial Fluid chemistry, Synovial Fluid drug effects, Uronic Acids metabolism, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Arthritis, Experimental drug therapy, Ceruloplasmin pharmacology, Regeneration physiology, alpha-Tocopherol pharmacology
- Abstract
The influence of administration of the antioxidant complexes consisting of nonenzymatic antioxidants (alpha-tocopherol acetate preparation) and enzymatic antioxidants (ceruloplasmin) has been studied in rabbits with experimental arthritis. The introduction of alpha-tocopherol acetate (at a daily dose of 4 mg) improved metabolic processes in the organism (decreased in the rate of erythrocyte precipitation, total leukocytes and their stub and segmental forms; increased in erythrocyte count; reduced the glycosaminoglycan content as determined from uronic acid and hexose level; decreased ceruloplasmin activity and malonic dialdehyde level ion blood serum, all at p < 0.05), thus favoring reduction in the total activity of the inflammatory process as judged from hematological and biochemical data. Intra-articular introduction of ceruloplasmin (1.5 mg/kg, once per week) positively influenced the state of joint structures in damaged knee joints of the animals: decreased the activity of ceruloplasmin (from 5.28 ± 0.06 to 3.94 ± 0.01 AU), and malonic dialdehyde level (0.18 ± 0.02 to 0.08 ± 0.01 μM) in the articular fluid (all at p < 0.05). These effects are probably related to the elimination of inefficiency of the antioxidant system in the synovial medium, thus preventing inflammatory destruction of articular tissues, hindering the development of pannus, and assisting the activation of reparative regeneration of connective tissue structures.
- Published
- 2015