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1. [Removal of the 3'- and 5'-untranslated region amplifies expression of the erythropoietin gene in mammalian cells].

Author

Park DKh, Kim C, Kim WB, Kim YK, Lee SY, and Yang JM

Subjects
Animals, Base Sequence, COS Cells, DNA Primers, DNA, Complementary, Polymerase Chain Reaction, RNA, Messenger genetics, Transcription, Genetic, 3' Untranslated Regions, 5' Untranslated Regions, Erythropoietin genetics, Gene Expression Regulation genetics
Abstract

The effect of the 5T- and 3T-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.

Published
2001

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