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The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.

Although alfalfa is the most important forage crop grown in the temperate regions, breeding improvements are not easily achieved due to high genetic complexity of this species. Because of allogamy and tetraploidy, cultivated alfalfa synthetic varieties have a high degree of genetic diversity among individual plants in populations. The knowledge of the extent of genomic variability in cultivars bearing desirable agronomic traits is an area that could benefit from molecular genetic analyses. Therefore, the aim of the present work was to evaluate the ability of RAPD markers to detect intra-population variation in fi ve alfalfa varieties. Presented here are the first results of investigating 40 randomly selected RAPD primers in order to establish whether they were able to synthesize PCR products, which would enable detection of polymorphism between the selected varieties and inside them as well.

ISSR method was applied for a detection of induced variability on a DNA level in M1 generation after Ethylmethan Sulphonate (EMS) chemical treatment in concentrations 0.5, 0.6 and 0.7% of seeds from head cabbage, Brassica oleraceae L, var. Capitata, cv. Ditmarsko. Different patterns of mutant plants obtained from the studied species were established by randomly selected primer sequences among tandem repeats. Polymorphisms were revealed amongM1 and control plants. Diverse DNA patterns of mutant plants were identified. As a result of the pre-breeding screening done in M1, altered plants were selected and seeds f or growing the next M2 generation were gathered.

Although alfalfa is the most important forage crop grown in the temperate regions, breeding improvements are not easily achieved due to high genetic complexity of this species. Because of allogamy and tetraploidy, cultivated alfalfa synthetic varieties have a high degree of genetic diversity among individual plants in populations. The knowledge of the extent of genomic variability in cultivars bearing desirable agronomic traits is an area that could benefit from molecular genetic analyses. Therefore, the aim of the present work was to evaluate the ability of RAPD markers to detect intra-population variation in five alfalfa varieties. Presented here are the first results of investigating 40 randomly selected RAPD primers in order to establish whether they were able to synthesize PCR products, which would enable detection of polymorphism between the selected varieties and inside them as well. [ABSTRACT FROM AUTHOR]