1. Proton to Carbon-13 INEPT in Solid-State NMR Spectroscopy.
- Author
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Elena, Bénédicte, Lesage, Anne, Steuemagel, Stefan, Böckmann, Anja, and Emsley, Lyndon
- Subjects
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PROTONS , *CARBON , *NUCLEAR magnetic resonance spectroscopy , *ORGANIC solid state chemistry , *ORGANIC compounds , *ORGANIC chemistry - Abstract
A refocused INEPT through-bond coherence transfer technique is demonstrated for NMR of rigid organic solids and is shown to provide a valuable building block for the development of NMR correlation experiments in biological solids. The use of efficient proton homonuclear dipolar decoupling in combination with a direct spectral optimization procedure provides minimization of the transverse dephasing of coherences and leads to very efficient through-bond 1H-13C INEPT transfer for crystalline organic compounds. Application of this technique to 20 heteronuclear correlation spectroscopy leads to up to a factor of 3 increase in sensitivity for a carbon-13 enriched sample in comparison to standard through-bond experiments and provides excellent selectivity for one-bond transfer. The method is demonstrated on a microcrystalline sample of the protein Crh (2 x 10.4 koa). [ABSTRACT FROM AUTHOR]
- Published
- 2005
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