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The rapid scientific and technological development of recent decades has led to digital technologies becoming an important part of the lives of the many people who use them to obtain information and find solutions to various problems. The most used digital technologies today include smartphones and mobile applications. Although mobile phones and the mobile applications used through them were not seen as learning tools for a long time, they have gradually found their way into schools with the aims of making teaching more attractive and to support pupils’ learning. In this context, there is a need for conceptual frameworks and methodologies that support the active and meaningful use of mobile applications to meet the stated educational objectives. By appropriately selecting mobile applications and embedding them into teaching, teachers can enable pupils to acquire knowledge, and to observe and explore various natural phenomena and processes that can be challenging to simulate in a classroom setting. Therefore, in this article, we focus on the presentation of the results of a quasi-experiment aimed at the implementation of mobile applications in the thematic unit “The Human and the Human Body” and their impact on the educational results achieved by pupils. The data obtained suggest that the use of mobile applications can have an impact on the level of knowledge of pupils in the seventh year of primary school. [ABSTRACT FROM AUTHOR]