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Abstract: The objective of this work was to evaluate the effect of raised seedbeds associated with irrigation on the yield of soybean (Glycine max) rotated with rice (Oryza sativa) in lowland conditions in Southern Brazil. Field experiments were conducted in two crop seasons (2014/2015 and 2015/2016), with two planting systems (raised seedbed and flat planting) and two irrigation managements (irrigated and nonirrigated). Water use, biological nitrogen fixation, and yield were evaluated. The water used for raised seedbeds was 14% (151 m3 ha-1) and 27% (163 m3 ha-1) lower than that for flat planting in the first and second crop seasons, respectively. Irrigation increased nodule number per plant, nodule dry weight, and biological nitrogen fixation. The average grain yield of the raised seedbed system was 10% (529 kg ha-1) and 9% (362 kg ha-1) higher than that of flat planting in 2014/2015 and 2015/2016, respectively. Irrigation improved yield by 5% (203 kg ha-1) and 7% (265 kg ha-1) in each crop year. The use of raised seedbeds associated with irrigation improves the yield of soybean grown in rotation with rice in lowland in Southern Brazil.