1. [Vascular morphological and microdensity changes of corneal neovascularization induced by topical bevacizumab and sunitinib in an animal model].
- Author
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Pérez-Santonja JJ, Campos-Mollo E, Lledó-Riquelme M, Fernández-Sánchez L, and Cuenca-Navarro N
- Subjects
- Angiogenesis Inhibitors pharmacology, Animals, Antibodies, Monoclonal, Humanized pharmacology, Arteries drug effects, Arteries ultrastructure, Bevacizumab, Corneal Neovascularization etiology, Corneal Neovascularization pathology, Disease Models, Animal, Drug Evaluation, Preclinical, Drug Synergism, Indoles pharmacology, Male, Platelet-Derived Growth Factor antagonists & inhibitors, Protein Kinase Inhibitors pharmacology, Pyrroles pharmacology, Rabbits, Sunitinib, Sutures adverse effects, Vascular Endothelial Growth Factor A antagonists & inhibitors, Veins drug effects, Veins ultrastructure, Angiogenesis Inhibitors therapeutic use, Antibodies, Monoclonal, Humanized therapeutic use, Cornea blood supply, Corneal Neovascularization drug therapy, Indoles therapeutic use, Protein Kinase Inhibitors therapeutic use, Pyrroles therapeutic use
- Abstract
Objective: To evaluate the effects of topical bevacizumab and topical sunitinib on vascular microdensity and morphology of corneal neovascularization (NV)., Methods: A total of 33 rabbits were distributed into 3 groups: group 1 (control; n=11): saline; group 2 (n=11): bevacizumab 5mg/ml; and group 3 (n=11): sunitinib 0.5mg/ml. A corneal NV model was used, based on sutures in the right eye of each rabbit. Each treatment was administered topically 3 times daily for 14 days. Corneas were then processed for the study of vascular microdensity (6 eyes) and vascular morphology analysis (5 eyes) using enzymatic staining histological techniques, Results: The vascular response in group 3 was limited to small-sized tree formations with various vascular axes compared with the extensive, lush and directional corneal NV of group 1 and 2. In the histological sections near the limb, there were no differences in vascular microdensity studies between the three groups. However, the mean sectional area of vessels (MSAV) in group 3 was 41.88% lower than in group 1 and 19.19% lower than in group 2. In distal sections, there were no differences between groups 1 and 2. However, group 3 was characterized by absence of vessels., Conclusions: Bevacizumab produced no changes in the morphology of the vessels or the vascular microdensity. Sunitinib reduced the size of the new vessels and induced changes in the vascular tree., (Copyright © 2012 Sociedad Española de Oftalmología. Published by Elsevier Espana. All rights reserved.)
- Published
- 2013
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