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New technologies in vaccinology are capable of achieving fast development, as well as large-scale production of effective and safe vaccines. Reverse vaccinology is an in silico methodology, which studies different characteristics of infectious agents, in order to identify antigens that are good vaccine candidates, without the need of traditional culture. This strategy is based on bioinformatics tools, that in a simple, safety and inexpensive way, reduces time and effort significantly in the new vaccine design, against traditional vaccinology. In recent years, the rapid spread of infections by emerging pathogens requires prompt development of new vaccines. Bioinformatic strategies joined with the latest next-generation vaccines allow the selection of vaccine candidates in a short time, which is relevant in the development of new vaccines against pathogens with pandemic potential.