1. Isolation of Mycobacterium gordonae from poultry slaughterhouse water in São Paulo State, Brazil Aislamiento de Mycobacterium gordonae de aguas de un abatidero de aves del Estado de São Paulo, Brasil
- Author
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K.A. Prince, A.R. Costa, A.C. Malaspina, A.F. Luís, and C.Q.F. Leite
- Subjects
Mycobacterium gordonae ,genotipos de PRA ,aguas de abatidero de aves ,PRA genotypes ,poultry slaughterhouse water ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Water samples (24 untreated water, 12 treated water and 24 served water) used in different stages of the slaughter process were examined to identify a possible source of pathogenic mycobacteria. The isolates were identified based on microscopy, morphological and biochemical features, mycolic acid analysis and molecular method - PCR-restriction-enzyme analysis. Eighteen mycobacterial strains were isolated from 60 water samples: 11 from untreated water, 5 from treated water and 2 from served water. All mycobacteria isolated were identified as Mycobacterium gordonae and showed the following PRA genotypes: III (27.8%), IV (38.9%) and V (33.3%).Muestras de aguas usadas en distintas etapas del proceso de matadero (24 de aguas no tratadas, 12 de aguas tratadas y 24 de procesos industriales) fueron analizadas con el propósito de evaluar posibles presencias de micobacterias patogénicas. Los aislamientos fueron identificados a través de microscopia, aspectos morfológicos y bioquímicos, análisis molecular (PCR-restriction-enzyme analysis) y de ácidos micólicos. Dieciocho aislamientos fueron recuperados de 60 muestras: 11 de aguas no tratadas, 5 de aguas tratadas y 2 de aguas utilizadas en procesos industriales. Todos ellos fueron identificados como Mycobacterium gordonae y mostraron los siguientes genotipos de PRA: III (27,8%), IV (38,9%) y V (33,3%).
- Published
- 2005