1. Use of New Techniques in Addition to IHC Applied to the Diagnosis of Melanocytic Lesions, With Emphasis on CGH, FISH, and Mass Spectrometry.
- Author
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Nagarajan P, Tetzlaff MT, Curry JL, and Prieto VG
- Subjects
- Antigens, Neoplasm analysis, Antigens, Neoplasm genetics, Biomarkers, Tumor genetics, Cell Differentiation, Humans, Immunohistochemistry, Melanocytes chemistry, Melanocytes pathology, Melanoma chemistry, Melanoma genetics, Melanoma pathology, Neoplasm Proteins analysis, Neoplasm Proteins genetics, Nevus, Pigmented chemistry, Nevus, Pigmented genetics, Nevus, Pigmented pathology, Prognosis, Skin Neoplasms chemistry, Skin Neoplasms genetics, Skin Neoplasms pathology, Staining and Labeling, Biomarkers, Tumor analysis, Comparative Genomic Hybridization, Dermatology methods, In Situ Hybridization, Fluorescence, Mass Spectrometry, Melanoma diagnosis, Nevus, Pigmented diagnosis, Skin Neoplasms diagnosis
- Abstract
Melanoma remains one of the most aggressive forms of cutaneous malignancies. While its diagnosis based on histologic parameters is usually straight forward in most cases, distinguishing a melanoma from a melanocytic nevus can be challenging in some instances, especially when there are overlapping clinical and histopathologic features. Occasionally, melanomas can histologically mimic other tumors and even demonstration of melanocytic origin can be challenging. Thus, several ancillary tests may be employed to arrive at the correct diagnosis. The objective of this review is to summarize these tests, including the well-established and commonly used ones such as immunohistochemistry, with specific emphasis on emerging techniques such as comparative genomic hybridization, fluorescence in situ hybridization and imaging mass spectrometry., (Copyright © 2016 AEDV. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2017
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