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Your search keyword '"Protein precipitation"' showing total 3 results
Start Over Descriptor "Protein precipitation" Language spanish; castilian
3 results on '"Protein precipitation"'

1. Optimization of a rapid method for screening drugs in blood by liquid chromatography tandem mass spectrometry

Author

Rodrigo Valero Alba M., Jorge Oscar Quintela, Serrano Begoña Bravo, and Tejedor Sara Ayuso

Subjects
screening, removal of phospholipids, mass spectrometry, protein precipitation, blood, Medical technology, R855-855.5
Abstract

In the recent years, liquid chromatography with tandem mass spectrometry has gained popularity in laboratories. This technique has a higher specificity, detects different analytes from a single specimen, measures analytes in distinct matrices, and substantially reduce analytical interference, with respect to immunoassay. The processing and preparation of biological samples are crucial in chromatography. Interferences in blood testing are usually caused by the presence of phospholipids and proteins. The main objective of this study was to improve analytical processes for drug screening by LC-MS/MS using a novel blood sample preparation method based on protein precipitation and removal of phospholipids.

Published
2023
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2. Padrões eletroforéticos das proteínas de reserva em grãos de sorgo com presença e ausência de tanino Electrophoretic patterns of grain storage proteins of sorghum with presence and absence of tannin

Author

Walter Alvarenga Rodrigues, Paulo César Magalhães, Edilson Paiva, and Fredolino Giacomini dos Santos

Subjects
Compostos fenólicos, método Azul da Prússia, precipitação de proteínas, Phenolic compounds, Prussian Blue assay, protein precipitation, Agriculture (General), S1-972
Abstract

Muitos métodos para determinar a presença de taninos são descritos na literatura, mas nenhum deles é universalmente aceito como ideal ou utilizado de forma unânime. Alguns métodos colorimétricos não diferenciam taninos de outros compostos fenólicos, outros utilizam substâncias que não são adequadas como padrão. Métodos que utilizam a capacidade dos taninos de precipitar as proteínas podem causar resultados divergentes devido às diferenças na conformação dessas moléculas. Assim, objetivou-se, neste estudo, identificar a presença de taninos em 10 híbridos de sorgo através da análise de padrões proteicos obtidos por eletroforese. O método colorimétrico Azul da Prússia foi utilizado para quantificar os taninos nas amostras. A precipitação das proteínas pelos taninos permitiu identificar os genótipos de sorgo com tanino através dos padrões proteicos das frações albuminas, globulinas e prolaminas. A análise eletroforética das prolaminas mostrou que as bandas produzidas pelo polipeptídeo kafirina, podem ser utilizadas na identificação de sorgo sem tanino no grão.Several methods are described on the literature to determine the presence of tannin, however none of them is universally accepted as the ideal or even are used in a unanimous way. Some colorimetric methods do not differentiate tannins from others phenolic compounds, others use substances which are not appropriate to use as standard. Methods that use the capacity of tannins to precipitate proteins may end up with divergent results due to differences on the molecule conformation. Thus, the objective of this study was to identify the presence of tannin in 10 hybrids of sorghum throughout analysis of pattern proteins obtained by electrophoresis. The colored method Prussian Blue was used to quantify tannins on the samples. The protein precipitation by tannins permitted to identify the sorghum genotypes with grain tannin through protein standards of fractions of albumin, globulin and prolamins. The electrophoresis analysis of the prolamins showed that the bands produced by the kafirin polypeptide may be used in sorghum identification without the presence of tannin in grain.

Published
2009
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3. Evaluación y tratamiento de efluentes del remojo convencional y enzimático de pieles, por precipitación de proteínas y coagulación

Author

Rocío Vargas P, Yasmín Liseth Castañeda C, Mary Flor Césare C, and Lizardo Visitación F

Subjects
coagulación, remojo convencional, tratamiento, conventional soaking, treatment, protein precipitation, Enzymatic soaking, General Earth and Planetary Sciences, Remojo enzimático, coagulation, precipitación de proteína, General Environmental Science
Abstract

Esta investigación evaluó y caracterizó efluentes del proceso de remojo convencional y enzimático de pieles, fue realizada a escala industrial con 20 pieles de ganado vacuno en las instalaciones de la empresa Helianthus S.A.C. Lima, Perú. Asimismo, se evaluó un sistema de tratamiento para dichos efluentes, constituido por precipitación de proteínas seguida de coagulación. Finalmente, el lodo seco de cada tratamiento se evaluó por separado para su potencial uso como fertilizante. La precipitación química de proteínasfue realizada aplicando un pH de 10, 11 y 12, usando KOH; mientras que en la coagulación se usaron dosis de 600, 800 y 1000 mg/L de sulfato de aluminio al 17%. En el remojo convencional, el sistema de tratamiento propuesto logró eficiencias de 70,3%; 84,9%; 92,4%; 93,4%; 61,8%; 93,9% y 99,6%; mientras que en el remojo enzimático, 80,5%; 83,8%; 93,3%; 93,8%; 59,3%; 93,5% y 99,4% en términos de DBO, DQO, SST, AyG, NTK, S2- y turbidez, respectivamente. El agua residual del proceso enzimático presentó mayor carga contaminante que el proceso convencional, probablemente relacionado a una mejor limpieza de las pieles por parte de la enzima Tanzyme RD04 utilizada. Para el tratamiento del efluente del remojo convencional y el enzimático, el pH óptimo de precipitación química fue 12 y la dosis óptima de coagulante, 800 mg/L. Los lodos residuales obtenidos presentan un alto contenido orgánico. This research consisted of the evaluation and characterization of wastewater generated during the leather soaking process, both in its conventional method and its enzymatic method. The experiments were conducted at an industrial scale at the Helianthus S.A.C. Company in Lima, using 20 cow hides. Likewise, a treatment process was evaluated for these effluents consisting of protein precipitation and was followed by coagulation. Finally, the dry sludge from each treatment method was evaluated separately for its potential use as fertilizer. The process for the chemical precipitation of proteins consisted of applying KOH until reaching pH values of 10, 11 and 12; while the coagulation process consisted of the application of doses of 600, 800 and 1000 mg/L of aluminum sulphate 17%. When using the conventional soaking process wastewater, the proposed treatment system achieved removal efficiencies of 70.3%, 84.9%, 92.4%, 93.4%, 61.8%, 93.9% and 99.6%; when using the enzymatic soaking process wastewater, the system achieved 80.5%, 83.8%, 93.3%, 93.8%, 59.3%; 93.5% and 99.4% removal efficiencies of BOD, COD, TSS, O&F, TKN, S2- and turbidity, respectively. The wastewater from the enzymatic soaking process had a higher level of pollutants than the one from conventional soaking, likely due to a better soaking of the hides with the Tanzyme RD04 enzyme which was used. For the treatment of effluents from the conventional as well as the enzymatic soaking process, the optimum precipitation pH was 12 and the optimum coagulation dose for both effluents was 800 mg/L. The organic content in the sludge was high.

Published
2016
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