Search

FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.

As compared with its normal oleate counterpart, high oleate peanuts have better storage quality and several health benefits, and are therefore preferred by peanut shellers and consumers. High oleate has now become one of the main breeding objectives of peanuts. Thus far, over 50 high oleate peanut cultivars have been registered. Yet high oleate peanut breeding relies heavily on a limited number of high oleate genotypes. In this paper, we reported, for the first time, high peanut oleate natural mutants with large seeds derived from an intersectional cross, which were identified with near infra-red spectroscopy and confirmed by gas chromatography. Sequencing of FAD2 from the high-oleic hybrids along with their normal oleate parents indicated that a 448G >A mutation in FAD2A coupled with a 441_442ins A or G in FAD2B together caused high oleate phenotypes in these peanut hybrids.

Objective: The organizing pneumonia (OP) pattern is the second most common finding in anti-synthase antibody syndrome (ASS), and nonspecific interstitial pneumonia (NSIP) is the most common finding. This study analysed the OP score changes by semiquantitative and quantitative analysis methods and the correlation between the high-resolution computed tomography (HRCT) indexes and the pulmonary function test parameters (PFTs) in ASS patients., Methods: Data from ASS-OP patients admitted to the respiratory department of Ping Jin Hospital from October 2014 to June 2020 were retrospectively reviewed and analysed., Results: Fourteen ASS-OP patients were recruited for this study. (1) In method-1, the consolidation (CO) score and the mean lung attenuation (MA) of poorly aerated and fibrosis lung fields (MA fibrosis ) (r=0.56, P=0.04), the ground-glass opacity (GGO) score and the MA of non-aerated lung fields (MA nonaerated ) (r=-0.64, P=0.01), and the CO plus the GGO (CG) score and the MA nonaerated (r=-0.59, P=0.03) of the lung fields had liner correlations. In method-2, the GGO score to the MA nonaerated (r=-0.58, P=0.03), and the CG (r=-0.68, P=0.01) score to the MA nonaerated had liner correlations. The FVC% (r=0.68, P=0.01) and FEV 1 % (r=0.64, P=0.01) to the MA fibrosis had good linear correlations. (2) Compared to the values before treatment, the CO pattern score, volume and weight percentages of the extracted whole lung volume with attenuation values of the nonaerated area (V nonated %, W nonaerated %), the volume of poorly aerated and fibrosis lung tissue (V fibrosis %, W fibrosis %), the weight percentages of normal aerated lung (W normal %), and the MA fibrosis exhibited significant differences during the 3-6 month follow-up period., Conclusion: The GGO and CO scored by the semiquantitative or quantitative analysis methods was similar. The HRCT quantitative analysis parameters showed a good correlation with the PFTs in ASS-OP patients, can provide an accurate OP pattern interpretation, and may be used as a monitoring and therapeutic indicator for ASS-OP patients., (Copyright © 2021 Elsevier España, S.L.U. All rights reserved.)