1. [Functional disorders of FOF1-ATPase in submitochondrial particles obtained from platelets of patients with a diagnosis of probable Alzheimer's disease].
- Author
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Martínez-Cano E, Ortiz-Genaro G, Pacheco-Moisés F, Macías-Islas MA, Sánchez-Nieto S, and Rosales-Corral SA
- Subjects
- Alzheimer Disease blood, Alzheimer Disease diagnosis, Blood Platelets cytology, Disease Progression, Female, Humans, Hydrogen-Ion Concentration, Male, Alzheimer Disease enzymology, Alzheimer Disease physiopathology, Blood Platelets enzymology, Proton-Translocating ATPases metabolism, Submitochondrial Particles enzymology
- Abstract
Introduction: Recent studies indicate that decreased energy generation by mitochondria is a feature that is common across neurodegenerative diseases., Patients and Methods: In order to obtain direct evidence that mitochondrial functioning is altered, we measured the hydrolytic activity of F0F1-ATPase and its capacity to generate a stable proton gradient in submitochondrial particles in 29 patients diagnosed with probable Alzheimer's disease (AD). Submitochondrial particles were obtained from platelets of patients with a diagnosis of probable AD and from clinically healthy controls., Results: Data revealed that the hydrolytic activity of F0F1-ATPase increases significantly in patients with probable AD (41.7+/-4.3 nmol PO4 min-1[mg protein]-1, n=29) as compared to the control subjects (29.1+/-1.9 nmol PO4 min-1 [mg protein]-1, n=29). It is important to note that, in the male population with probable AD, we found that hydrolytic activity of F0F1-ATPase increased as cerebral deterioration progressed. We also detected a lower pH gradient in the submitochondrial particles of patients with probable AD (0.28+/-0.08 pH units, n=25) as compared to the controls (0.5+/-0.1 pH units, n=20)., Conclusions: Overall, these data point to an alteration in the functioning of the enzyme.
- Published
- 2005