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Start Over Author "W P, Yang" Language spanish; castilian
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1. An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.)

Author

H. T. Yu, W. Q. Yang, Y. Y. Tang, X. Z. Wang, Q. Wu, D. Q. Hu, C. T. Wang, and S. L. Yu

Subjects
allele specific pcr (as-pcr), fad2a/fad2b, genotyping, peanut, Nutrition. Foods and food supply, TX341-641
Abstract

FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.

Published
2013
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