In this paper the results of the evaluation of an instrument on gender institutional culture are analyzed using the principal components method. The structure and parameters of its validity and reliability, as well as the differences obtained by sex, are described. The sample consisted of 1,144 individuals from the educational, health, and research sectors in northwestern Mexico. The results showed four components: administrative processes, employment equity, benefits, working environment, and authoritarianism, which explain 40.90 of variance with a Cronbachs alpha of .88. With these obtained values, the instrument is considered valid and reliable to evaluate institutional culture and identify gender bias. Methodology can be replicated to adapt the instrument to other regions. [ABSTRACT FROM AUTHOR]