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The research was carried out in October, 2011 and March, 2012. Follow image satellite analyses 41 sites were selected in Ana Maria gulf, south region of Cuba. At these sites an inventory of the corals, gorgonians and sponges were carried out. Health status was analysed for corals. Inventories were carried out in mangrove, seagrass and coral reefs (patch reefs and sand-rocky bottom biotopes) ecosystems. Species richness for corals was 41, 23 for gorgonians and 41 for sponges. More abundant corals species were Porites astreoides, Millepora alcicornis, Siderastrea siderea, Siderastrea radians, Agaricia agaricites and higher diversity was detected in patch reefs (41 species). More abundant gorgonians were Gorgonia ventalina, Erythropodium caribaeorum, Briareum asbestinum and the sponges were Mycale laevis, Cliona aprica, Iotrochota birotulata. Main affectation to coral health was bleaching (21 species experienced bleaching in some degree and only in 51% of sites). In Rabihorcado key and Algodón Grande key, were detected higher recruit abundance. Higher corals species richness was observed in: Algodón Grande key, Manuel Gómez key, Bergantines and Palomo keys. Having these results as base, this paper proposes research corals priorities relate to growth of colonies, connectivity among populations and health status of such populations in Ana Maria gulf-Jardines de la Reina ecosystem. [ABSTRACT FROM AUTHOR]