Search

The aim of our study was to investigate the effect of inflammation in the placenta on the pro-apoptotic development after severe preeclampsia. Placenta tissue samples of 15 HELLP syndrome and 15 healthy 35-38th week-pregnant women were involved in the study. Tissue samples were taken only from the maternal side of the placenta and fixed in 10 % formaldehyde, then blocked in paraffin wax and 4-6 mm-thick sections were cut and stained with Harris Hematoxylene-Eosin. Antigen retrieval was performed for sections, incubated with FAS antibody and anti-IL-6 antibody. After the application of streptavidin peroxidase followed by AEC chromogen solution, sections were counterstained with Harris hematoxylin. Significant thickening of the fibrinoid layer, degeneration and apoptotic change in decidua cells, marked increase in the hyalinized area, degenerative changes in the syncytial regions of the chorionic villus and an increase in syncytial nodes and bridges and IL-expression were observed as positive. FAS expression was positive in the pycnotic nuclei of decidual cells in the maternal region and in the syncytial regions. It was observed that the proapoptotic process increased as a result of severe preeclampsia. It was concluded that the control of cytokine activity and reduction of pro-apoptotic signal during the inflammation process will slow down the development of HELLP syndrome. [ABSTRACT FROM AUTHOR]

Objective. To evidence of the presence of two species of monogeneans of the genus Cichlidogyrus that cause damage to gill tissue, in tilapia Oreochromis spp. grown in Ecuador. Materials and methods. The gills were placed in a Petri dish with boiling water so that the parasites relax, and later they were fixed in 4% formalin. The samples were checked under a stereoscope microscope. The parasites were counted and preserved in 70% ethanol for their taxonomic identification according to their morphological characteristics. The infection parameters (prevalence, mean abundance and mean intensity) were calculated for each species of parasite. For the description of histological damages, fragments of parasitized gills were fixed in 10% neutral formalin and processed by the histological paraffin embedding technique. Results. Cichlidogyrus sclerosus showed highest infection parameters, prevalence of 65%, mean abundance of 24.24 ± 68.62 and mean intensity of 37.62 ± 82.93. Cychlidogyrus dossoui was observed in a prevalence of 4.5%, mean abundance of 0.13 ± 0.66 and mean intensity of 3 ± 1.41. Both species caused hyperplasia in lamellae. Conclusions. This study constitutes the first report of infestation by Cichlidogyrus sclerosus and C. dossoui in tilapia culture systems of Ecuador. [ABSTRACT FROM AUTHOR]

Introduction: Ornamental Angel fish (Pterophyllum scalare) is one of the main commercialized species from South America, with an important number of national and international dealers. Objective: To make a histological and morphometric description of P. scalare digestive tract. Methods: Nineteen adult individuals were used. Intestinal coefficient values were obtained, and a macroscopic and microscopic description of esophagus, stomach, and intestine (anterior, middle and posterior) was carried out. Samples were processed for paraffin embedding, and 5 µm sections were stained with H&E and P.A.S. Positive P.A.S. cells were counted. Lumen area and tissue area were measured in representative digitalized sections from each region. Results: P. scalare exhibited a short esophagus, followed by a blind bag shaped, scarcely muscular stomach, and a short intestine (intestinal coefficient 1.66 +/- 0.52). Four constitutive tissue layers were identified: mucosa, submucosa, muscular and in some areas also a serosa layer. Esophagus exhibit abundant globet P.A.S. + cells and shows the larger tisular area was observed. Luminal area and perimeter are larger in anterior intestinal region. Conclusions: the intestinal coefficient value (1.66 +/- 0.52), and the histological and morphometric characteristics of the evaluated organs, suggest that P. scalare is omnivorous with soft prey preferences. [ABSTRACT FROM AUTHOR]

Thermal accumulation facilities allow energy to be available in the absence of sunlight. This fact reduces the difficulty of the intermittence in the incidence of the king star in our planet. Thermal accumulation technology also contributes to smooth the fluctuations in energy demand during different times of the day. This contribution identifies the nations with the most favorable research results in this area; as well as the main research lines that are being developed today. A compendium of various thermal energy storage materials, their current costs per unit mass, and their physical properties are presented. Techniques for implementing thermal accumulation technologies can be classified as areas of high, medium and low temperature. In the high temperature area, inorganic materials such as nitrate salts are the most widely used thermal energy storage materials, while in the medium and lower temperature areas; organic materials such as commercial paraffin are more common. Currently, one of the research trends in this area are the projects aimed at optimizing the chemical and physical characteristics of thermal storage materials, because the success of any thermos-energetic storage technology has a strong dependence on the cost of the materials selected for thermal storage. [ABSTRACT FROM AUTHOR]

In 2009, in the Tepeaca Valley, Puebla, it was found a cyst-forming nematode of the genus Heterodera associated with carrot. At this time, it is unknown the distribution in some producing municipalities, its life cycle and the histological changes on infected plant roots. Between 2008 and 2016, 28 carrot fields were sampled in six municipalities of the Tepeaca Valley. The roots were separated and white females were searched, the cysts were extracted from soil. Carrot cv. Mexicana were grown in naturally infested soil in the greenhouse, the roots were stained with fuchsine lacto glycerol acid to know the development time of each cycle phase. To describe the histological changes, the infected roots were imbibed in paraffin and sections stained with fast green-fuchsine for analysis. Heterodera sp. was found in 93 % of the plots and completed its cycle in 73 days at 20-25 °C. The histological alterations show the presence of feeding sites (syncytia) with dense and granular cytoplasm, with hypertrophied nuclei, dissolution and thickening of cell walls located mainly in the cortex and vascular cylinder. There was also the presence of lignificate areas with hyperplasia and hypertrophy. [ABSTRACT FROM AUTHOR]

Cassava roots are very susceptible to physiological deterioration; after a few days of harvesting (two or three) they are not acceptable for consumption. For this reason, diverse methods have been developed for their preservation, for example, coating them with paraffin, which prolongs the product's shelf life up to 15 days. This conservation method with paraffin has some disadvantages from the environmental point of view because this coating is a hydrocarbon derived from petroleum and it is applied hot to the roots (temperatures above 120 °C). Thereby, it is important to seek alternatives of environmentally friendly preservation. This work evaluated the effectiveness of root coating with natural wax as a possible substitute for paraffin for the conservation of the product under natural environmental conditions. The results showed that natural wax is as effective as paraffin in preserving cassava roots; it delays physiological deterioration, as well as weight and dry matter losses. [ABSTRACT FROM AUTHOR]

Histologically, the adult human cerebellar cortex has three constituent layers, the molecular layer, the row of bodies of Purkinje cells and internal granular layer. The cortex of the cerebellum of mammalian embryos, as well as youth, show external granular layer. Our goal is to document morphological differences between the layers of the cerebellar cortex adult and young. Microscopic morphometric studies were performed in 20 cerebellum autopsied. The cases were divided into two groups, adults and children under 1 year. Paraffin sections were performed, stained with hematoxylin and eosin. The cortex of adult cerebellum showed morphological differences of the newborn. In the adult cerebellum has three constituent layers, whereas in the newborn has a fourth layer, the external granular. [ABSTRACT FROM AUTHOR]

Se observaron estructuras quísticas similares a xenomas con organismos ovoides o piriformes en encéfalo y riñón de un conejo de un mes de edad. Se probaron diferentes tinciones, como HE, PAS, azul de toluidina y azul de tricromo. El azul de tricromo proporcionó los mejores resultados en cuanto a especificidad, resolución y confirmación de esporas de microsporidios. Este colorante es fácilmente adaptable a cortes desparafinados por no requerir de equipo adicional; es rápido, económico y podría utilizarse como técnica confirmatoria para otras microsporidiosis. [ABSTRACT FROM AUTHOR]

La extracción de ADN de tejidos embebidos en parafina (TEP) es crucial en muchos estudios moleculares. Varios han sido los protocolos utilizados para conseguir tal propósito. En esta investigación se compararon tres métodos de extracción de ADN con la finalidad de seleccionar uno para el trabajo en nuestro laboratorio. Se tomaron diez ganglios linfáticos de diez fallecidos por sida, para su estudio histológico y bacteriológico. El ADN se extrajo por calentamiento a 100 °C (método A); con el empleo de resina quelante Chelex-100 (método B) y por digestión con proteinasa K (método C). Para la identificación de Mycobacterium tuberculosis se amplificó una región de la secuencia específica de inserción IS 6110, mediante la reacción en cadena de la polimerasa. El ADN de los TEP se obtuvo degradado, con diferencias significativas de pureza. Solo se logró amplificación con los métodos B y C; este último fue el más laborioso. El método que requirió resina quelante Chelex-100 (método B) fue el más útil: se obtuvo ADN con calidad y cantidad suficiente en un menor tiempo. Por tanto, este método puede ser considerado como una buena opción en patología molecular. [ABSTRACT FROM AUTHOR]

The need to replenish wrinkles and depressions has enticed the creation of multiple solid or semi-solid injectable substances. The disastrous results of some of these have led me to create the term "Iatrogenic Allogenosis": "Allogenosis", because it is produced by allogenic (foreign) substances. "Iatrogenic", because we, the physicians or persons injecting these substances, have caused this disease. Over one million persons in Latin-America have become victims of these substances. Our objective is to learn how to treat these cases. We have studied 358 cases in a 10-year time span. We have grouped them and tried to reach conclusions. Mostly used substances are: liquid silicon, paraffin, liquid petrolatum, Vaseline, mineral oil, animal fat, etc. We have not included in this study some substances that have been fairly accepted by surgeons: autogenous fat, bovine collagen, hydroxyapatite, acrylic, hyaluronic acid, etc. Reactions are local and general. The latency period varies from 6 hours to 25 years. Local reactions are: pain, erhytema, edema, pigmentations, swelling, fibrosis, keloids, infection, fistulas, necrosis, gravity displacement, etc. The general ones are: fever, arthralgia, general discomfort, we have no find relations with autoimmune diseases (dermatomyositis, scleroderma, etc.). Treatment is symptomatic. Surgical resection has to be very limited because it leaves very notorious scars and retractions. Large masses must not be operated. Liposuction is not effective. Corticoids an massages make the situation even worst. It does not dissolve the masses and does atrophy the normal skin covering them, and thus, underlying masses are more noticeable. Exacerbation may occur every 3 or 4 months and will cure wit or without an anti-inflammatory or an antibiotic. An intense preventive campaign must be launched. [ABSTRACT FROM AUTHOR]

In this paper, we carry out a characterization of an emulsion of sugar cane wax in water obtained with the employment of a soap solution of fatty acids as emulsifying agent. It is also compared with the emulsion of paraffin, used as an hydrophobic agent in the production of bagasse boards, in order to study the possible substitution of the latter. The aspects considered in this characterization are the following: type of emulsion, stability and reological behavior. The latter has a great practical importance related to the emulsions. [ABSTRACT FROM AUTHOR]

The construction of copper (II) ions sensor of heterogeneous solid membranes is discussed, as electroactive substance to utilize a mixed copper (II) sulfide and silver (I) sulfide, and as agglutinating paraffin, for comparison of the literature shows an electrode most simple and resistant to this class of membranes. In this work presents some parameters for this types of electrodes such as: nernstiana response, practical limite of detection, selectivity coefficients for some ions metals and pH effect. [ABSTRACT FROM AUTHOR]

In this work the soaps fraction obtained as by-products in the extraction process from sterols are applied as an emulsification agent in the formulation of wax-water emulsions. Besides, these emulsions are used to substitute the paraffin emulsion in the manufacture of bagasse checkerboards. Several sets of experiments were carried out in order to evaluate the influence of the wax percent in the emulsion over the expansion percent of the checkerboards in water-parameter established by the Cuban procedures to evaluate the quality of the bagasse checkerboards. Other properties showing satisfactory results and demonstrating the feasibility of the substitution of the paraffin (high price in the international market) by the cane wax (national by-product) were determined as well. [ABSTRACT FROM AUTHOR]