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Introduction: the need to have rapid, sensitive and specific laboratory techniques for the detection of pathogens that are difficult to diagnose led to the creation of the Molecular Biology Laboratory at the Provincial Center of Hygiene, Epidemiology and Microbiology of Villa Clara. Objective: to characterize the evolution and importance of Molecular Microbiology in the Laboratory of the Provincial Center of Hygiene, Epidemiology and Microbiology of Villa Clara from June 2018 to December 2023. Methods: cross-sectional descriptive study carried out at the Villa Clara Molecular Biology Laboratory in the period from June 2018 to December 2023 based on the total samples processed by polymerase chain reaction, several technological platforms and various kits were used. commercial. Results: In 2018, molecular diagnosis was implemented with two platforms, Rotor Gene-Q® and Gene Xpert® MTB/RIF, and in 2021, four were available, when the SLAN and the COBAS 4800® platform were introduced. The laboratory became relevant due to the arrival of the 2019 new coronavirus pandemic: 511,225 nasopharyngeal exudate samples and 14,568 determinations were processed for the diagnosis of human immunodeficiency viruses, hepatitis C and B, and human papilloma carried out on the COBAS 4800® platform. In 2023, the pathogen detection capacity was strengthened and the community cocirculation of influenza A and B, parainfluenza virus type 4, the OC 43 coronavirus, SARS-CoV-2 and dengue was identified. Conclusions: the progressive increase in the resolution capacity of the Molecular Biology Laboratory, together with the implementation of new diagnostic platforms, has allowed us to strengthen the detection of pathogens of clinical interest and improve virological sentinel surveillance. [ABSTRACT FROM AUTHOR]

The objective of the work was to study stomatal characters, chlorophyll and their phylogenetic relationship in 17 cultivars of Musa spp. (AAB; ABB; AAA; AA), of the Germplasm Bank of la Universidad Técnica Estatal de Quevedo. The experiments were carried out in the Laboratory of Microbiology and Molecular Biology. Two methods of morphometric and physiological leaf evaluations are used: a) From leaf tissue of 25 cm2 the abaxial and adaxial stomatal density was evaluated in (e/mm2 ) and b) From leaf disks of 1,3 cm2 , was determined the total chlorophyll content. In molecular characterization by RAPD, were used five oligonucleotides. Significant statistical differences were determined (p ≤ 0,05) for the variables, with results of higher abaxial stomatal density in Barraganete of 233 e/mm2 and its directly proportional relationship between stomatal density and length, where the greater the density, the shorter its length. The chlorophyll content of 4,61 mg/g in Guineo seda. The phylogenetic tree generated two large groups at its genomic accession. Sub-group A brought together nine accessions to the genome (AAB), Sub-group B with five accessions of (AAA), highlighting Subgroup C containing the cultivar Orito of the genotype (AA) and Sub-group D with the accessions (AAB and ABB). [ABSTRACT FROM AUTHOR]

Background: Burkholderia cepacia (B. cepacia) has been the causal agent of infectious hospital outbreaks. Methods: a case-controls study was conducted to describe an outbreak of urinary tract infection associated to lubricant-gel contaminated with B. cepacia. Patients who developed UTIsc were included in this study. Their clinical data, urine cultures, antiobiograms and the molecular bacterial analysis were analyzed. Results: the urine culture was positive in 101 (55.8 %); the bacterial strains isolated were: B. cepacia 30.7 %, Escherichia coli 22.7 %, Enterococcus faecalis 9.9 %, Enterococcus spp. 8.9 %. They were 31 patients with B. cepacia isolation that was taken as the cases group and 63 with UTIsc due to another organism as control group. The Charlson comorbidity index was 5.3 ± 1.8 for cases and 4.5 ± 1.2 for controls. The factors associated with death were: heart disease, diabetes, pneumonia, bac-teremia and UTIsc B. cepacia. Conclusions: the lubricant gel used for urinary catheter placement originated the outbreak. Heart disease, pneumonia, bac-teremia and UTIsc B. cepacia were related to patients' death. [ABSTRACT FROM AUTHOR]

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