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Among the factors currently identified in the appearance of acne is the bacterial proliferation of Cutibacterium acnes due to seborrhea and hyperkeratosis. Topical acne treatments are strictly necessary and range from antibiotics to salicylic acid depending on the type of acne to be treated. The main mechanisms of action of topical products for this disease are aimed at eliminating excess bacteria from the skin and reducing the process of redness and inflammation. For the treatment of this disease, two creams were developed using two concentrations of topical OLEOZON® as active pharmaceutical ingredient, with polysorbate 80 as emulsifying agent and other excipients. A stability study was performed for six months, which showed its behavior at the two storage temperatures (2-8˚C and 30˚C) from the organoleptic, physicochemical and microbiological point of view, the latter showed a marked antimicrobial activity and absence of microorganisms in the samples during the six months of study. The organoleptic properties showed better results at low storage temperatures, since they remained uniform in terms of color, odor and texture; likewise, the physicochemical evaluation corroborated a better performance for the creams stored at low temperatures. [ABSTRACT FROM AUTHOR]

The healing of wounds that occur on the skin, after the occurrence of some traumatic action, constitutes a dynamic process characterized by the integration of four sequential phases based on hemostasis. There are different factors that affect wound healing; such is the case of bacterial infections and even metabolic diseases such as diabetes mellitus. Talking into account that there is little evidence of effective pharmacological therapies for the treatment of wounds and/or ulcers in the skin, the search for new pharmacotherapeutic strategies is a current topic. For this purpose, the use of ozonated oils has shown promising effectiveness as the treatment of choice to accelerate wound healing. So the objective of this work is aimed at identifying the ozonated oils used in the treatment of wound healing of the skin and their healing action mechanism. For this, all the published information that was available in the PubMed database was collected. In this sense, a brief description of the healing process, the fundamental factors that the control, the general factors of ozonized oils and the mechanisms of action described for their healing action in different experimental models were made. This document summarizes in an organized way all the results that ensure and sustain the effectiveness of ozonized oils, as stimulators of the healing process. It is concluded that ozonated oils facilitate the restoration of damaged skin through the activation of different molecular mechanisms. [ABSTRACT FROM AUTHOR]

The action mechanism of ozonized vegetable oils on microorganisms is still unknown. According to the oxidant nature of its main components, damages are expected on unsaturated lipids and proteins containing sulfhydryl groups of microorganisms. There is no evidence of effects induced by ozonized vegetable oils on bacterial cell membrane, which is one of the primary attack sites of several antimicrobial agents with a high oxidant power. The aim of this work was to evaluate the effect of ozonized sunflower oil (OLEOZON®) on Staphylococcus aureus ATCC 25923 strain, considering viability, cell membrane permeability and possible ultrastructural changes. At 1, 3, 10, and 30 min of exposition to OLEOZON® a microorganism susceptibility study was done. The content of potassium ions released to media by bacterial cells was employed as a criterion of alteration of the cell membrane permeability. Analysis of transmission electron microscopy to samples exposed to OLEOZON® during 30 min and 24 h were applied to evaluate possible ultrastructural changes. OLEOZON® showed a strong bactericidal effect on Staphylococcus aureus ATCC 25923 strain, which was manifested in a reduction of the viability of more than 90 %, since the first minute of exposition. OLEOZON® modified the cell membrane permeability of Staphylococcus aureus strain since a reduction of intracellular potassium content took place after the lost of cell viability. This drug altered Staphylococcus aureus ultrastructure causing a reduction of cytoplasmic content; nevertheless it did not affect the bacterial cell envelope integrity. The intensity of changes in viability, cell permeability and ultrastructure on Staphylococcus aureus increased with the exposure time to OLEOZON®. [ABSTRACT FROM AUTHOR]

Streptococcus mutans is considered the main ethiological agent of buccal caries. The aim of this work was to measure the lethality action of ozonized sunflower oil (OLEOZON®) on this microorganism. The influence of different conditions, like pH, agent concentration and contact time was evaluated. The values of minimum inhibitory concentration (CMI) were determined. The CMI shows similar behaviour among species of the same genus and different results for bacterias from other gender. It was proved that OLEOZON® activity did not vary significantly with the studied variation of pH, although the microorganism number decreases to pH lightly acid the highest decreased of initial microorganism concentration was about 99.9 %. The study of the influence of the different agent concentrations showed a significant effect of OLEOZON® in the microorganism lethality, being 285 mg/mL the most effective studied concentration. A ten-minute exposition time, between the agent and the microorganism, produces a 99.9 % of microorganism lethality; however with only three minutes of exposition time a reduction of 95.5 % of initial microorganism concentration was obtained. An equation which relates the study of three main physico-chemical parameters was obtained. This equation shows the influence of pH, antimicrobiological agent and exposition time upon of the inactivation of the microorganism. These results proved that OLEOZON® is an effective drug to treat Streptococcus mutans infection. [ABSTRACT FROM AUTHOR]