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This study reports the composition of 13 cultivars sesame seed that cultivated in Thailand using gas liquid chromatography equipped with mass spectrometer (GC-MS). The cholesterol, lanosterol and β-sitosterol were found to be highest in black sesame cultivar Nakornsawan with content of 6304.0±75.6, 300.0±3.2 and 10,072.0±99.7 mg/gram of sample, respectively. The content of stigmasterol and avenasterol were found to be highest in black sesame cultivar Chaibadan with content of 490.0±19.8 and 2200.0±136.4 mg/gram of sample, respectively. Moreover, black-red sesame cultivar Ubonrachatani 1 showed the highest campesterol and campestanol with content of 3744.5±81.9 and 490.0±19.8 mg/g sample, respectively. Beside the highest content of zymostanol and sitostanol was found in white sesame with content of 89.6±0.1 and 249.0±0.5 mg/g of sample. From all of these evidences support that the hypolipidemia effect of sesame is from not only the lignan compound but also sterol and stanol. [ABSTRACT FROM AUTHOR]