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In this study, nitrate removal was investigated in a packed bed bioreactor with Paracoccus denitrificans cells immobilized in calcium alginate. For this purpose, the effects of nitrate content and flow rate of the feed solution on biological denitrification were investigated. As a result of this examination, the nitrate concentration of the product water at the reactor outlet was found below the standards allowed by the World Health Organization and TS-266 up to a flow rate of 150 mL h-1. Maximum reactor efficiency was obtained as 31.53 mg L-1 h-1 at a feed solution flow rate of 150 mL h-1. The hydraulic retention time for this value is 30 minutes. The nitrate content of the feed solution was up to 250 mg L-1, and the nitrate concentration in the effluent was below the standards. Maximum nitrate removal rate and reactor efficiency were found as 342.06 and 30.70 mg L-1 h-1 at a loading rate of 397.50 mg L-1 h-1, respectively. [ABSTRACT FROM AUTHOR]