1. Fluorescence quenching study of moxifloxacin interaction with calf thymus DNA
- Author
-
Pan Li, Miaolun Jiao, Bao-Sheng Liu, Chao Yang, and Yun-Kai Lv
- Subjects
Quenching (fluorescence) ,Aqueous solution ,Hydrogen bond ,Chemistry ,Moxifloxacin,ct-DNA,fluorescence quenching,binding ,General Chemistry ,Photochemistry ,Binding constant ,symbols.namesake ,Moxifloxacin ,symbols ,medicine ,van der Waals force ,Binding site ,medicine.drug ,Antibacterial agent - Abstract
Moxifloxacin (MOX) is a fourth-generation synthetic fluoroquinolone antibacterial agent with many important therapeutic properties. Fluorescence quenching was used to study the interaction of MOX with calf thymus DNA (ct-DNA) in aqueous solution. The intercalative binding mode and a static quenching mechanism were confirmed by the Stern--Volmer quenching rate constant (Kq) of 3.48 \times 1011 M-1 s-1 at 298 K. The thermodynamic parameters (D H = --118.4 KJ mol-1 and D S = --299.4 J mol-1 K-1) were calculated at different temperatures, and they indicate that the main forces between MOX and ct-DNA are hydrogen bonding and Van der Waals force. We proved at the same time the presence of one single binding site on ct-DNA, and the binding constant is 1.28 \times 105 M-1 at physiological pH. The results may provide a basis for further studies and clinical application of antibiotics drugs.
- Published
- 2014