1. Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T
- Author
-
Dilsat Nigar Colak, Ali Osman Belduz, Derya Yanmis, Hakan Karaoğlu, Fulya Ay Sal, Sabriye Canakci, RTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümü, and Karaoğlu, Hakan
- Subjects
Xylose isomerase ,inorganic chemicals ,Physiology ,Characterization ,Anoxybacillus ,medicine.disease_cause ,Microbiology ,Divalent ,chemistry.chemical_compound ,Column chromatography ,Thermophilic ,Genetics ,medicine ,Molecular Biology ,chemistry.chemical_classification ,Xylulose ,Xylose isomerase,Anoxybacillus,characterization,thermophilic ,Cell Biology ,Enzyme ,chemistry ,Biochemistry ,Xylose isomerase activity ,General Agricultural and Biological Sciences ,Anoxybacillus gonensis - Abstract
BELDUZ, Ali Osman/0000-0003-2240-7568; WOS: 000341523900005 The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2(T) has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography techniques to homogeneity, and the biochemical properties of the enzyme were characterized. the optimum temperature of the enzyme was 85 degrees C and maximum activity was observed at a pH of 6.5. Its Km and Vmax values were calculated as 25 +/- 2 mM and 0.12958 +/- 0.002 mu mol/min/mg protein, respectively. the effects of various metal ions on the xylose isomerase were examined. Divalent cations Co2+, Mg2+, and Mn2+ were essential for xylose isomerase activity; however, bivalent metal ions (Ca2+, Hg2+, Ni2+, Zn2+, Fe2+, and Cu2+) showed inhibitory effects. This is the first report of characterization of the xylose isomerase of Anoxybacillus spp. According to results obtained from this study, xylose isomerase is a promising candidate for industrial applications in production of xylulose and ribose. Karadeniz Technical University Research Foundation [2003.111.04.6]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [104T472, TBAG-AY/395 (104T380)] This study was financially supported by the Karadeniz Technical University Research Foundation (Grant no. 2003.111.04.6) and the Scientific and Technological Research Council of Turkey (TUBITAK) (Grant no. 104T472 and TBAG-AY/395 (104T380)).
- Published
- 2015